Protein Mass Spectrometry
The Sigma-Aldrich enzyme portfolio provides a comprehensive and versatile toolkit for proteomics mass spectrometry workflows. Solutrypsin, a highly purified and soluble form of trypsin, offers consistent and efficient cleavage at the carboxyl side of lysine and arginine residues. Its superior solubility and reduced autolysis make it ideal for high-throughput and reproducible digestions, especially in complex or denaturing sample conditions. ASP-N complements this by targeting the N-terminus of aspartic acid residues, expanding sequence coverage in acidic regions often missed by trypsin alone. Glu-C adds further depth by cleaving at glutamic and aspartic acid residues, supporting detailed mapping of post-translational modifications. Lysarginase, which cleaves after lysine and arginine with a different specificity profile than trypsin, offers orthogonal cleavage patterns that enrich peptide diversity and proteome coverage. Together, these enzymes enable high-resolution, in-depth proteome analysis across a wide range of experimental conditions.
Products
Trypsin from bovine pancreas
TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
Trypsin Protease
Hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues, suitable for mass spectrometry, from Porcine pancreas
α-Cyano-4-hydroxycinnamic acid
suitable for MALDI-TOF MS
SILu™MAB K1 Stable-Isotope Labeled Universal Monoclonal Antibody
recombinant, expressed in CHO cells
SAvPhire™ Monomeric Streptavidin
High-affinity biotin binding—reduces aggregation and enables precise, reliable detection and purification of biotinylated molecules
SILu™MAB Stable-Isotope Labeled Universal Monoclonal Antibody Standard human
recombinant, expressed in CHO cells
SILu™MAb Adalimumab Stable-Isotope Labeled Monoclonal Antibody
SigmaMAb Antibody Drug Conjugate (ADC) Mimic
Antibody Cysteine-Fluorophore Conjugate Standard
Mucinase StcE Protease
cleaves mucin domains by recognizing specific peptide and glycan motifs and suitable for mass spec glycoproteomics
Trypsin Protease
Cleaves C-terminal of lysine and arginine residues, Suitable for Mass Spectrometry, from bovine pancreas
PNGase F Glycosidase
Removes N-linked glycans, suitable for mass spectrometry, from Elizabethkingia miricola
Trypsin Protease
Hydrolyzes peptide bonds at the carboxyl side of arginine and lysine residues, suitable for mass spectrometry, recombinant, expressed in Pichia pastoris
Enhanced Microbial Transglutaminase (eMTG)
Glycosylation tolerant, for site-specific antibody bioconjugation
SILu™Lite SigmaMAb Universal Antibody Standard human
Asp-N Protease
Cleaves N-terminal to aspartic acid residues, suitable for Mass Spectrometry, from Pseudomonas fragi mutant strain
Proteomics Dynamic Range Standard Set
Protein Mass Spectrometry Calibration Standard
SOLu-Trypsin Protease
Ready-to-use stable solution for automation, suitable for mass spectrometry, recombinant, expressed in Pichia pastoris
Glu-C Protease
Cleaves at glutamic acid residues, suitable for Mass Spectrometry, from Staphylococcus aureus V8
Protein A Turbo ID
Off-the-Shelf proximity biotinylation, suitable for mass spectrometry, recombinant
PNGase F Glycosidase
Removes all N-linked glycans, suitable for mass spectrometry, from Elizabethkingia meningosepticum
Enzyme Reagents
Reliable and efficient protein digestion with selective and pure sequencing grade proteases is a crucial step when preparing proteomics samples. We offer a wide range of both natural source and recombinant proteases including endoproteinases like Trypsin (T1426), Chymotrypsin (C6423), Lys-C (324715), Glu-C (324713), Asp-N (324708), as well as a variety of Aminopeptidases (A8200) and Carboxypeptidases (C9584). Additionally, SOLu-Trypsin is a ready-to-use recombinant trypsin solution (EMS0004). This Advanced Proteomics Grade enzyme is solution stable for mass spectrometry, designed to be stable in solution when refrigerated, and SOLu-Trypsin can be used immediately without preparation.
Silu™tions Stable Isotobe-Labeled Ms Internal Standards
We provide a wide range of proteomics standards designed specifically for mass spectrometry applications. Highly characterized protein and peptide mixtures are available for applications ranging from MS instrument performance to specific post translational analysis. All products are produced with the highest levels of quality and are designed specifically for your proteomics applications.
Our Universal Proteomics Standards (UPS) with well-defined, well-characterized standards are made up of 48 proteins 6-83 KD or 6 mixtures of 8 proteins (UPS2). Additionally, the MS Qual/Quant QC Mix (MSQC1) contains 6 trypsin digested human proteins and 14 SIL peptides, while the MS Retention Time Calibration Mix (MSRT1 and MSRT2) are injection-ready mixtures containing 14 stable isotope-labeled peptides or 9 native proteins, respectively.
SILu™Prot MASS SPEC PRODUCTS
SILu™Prot products are a novel collection of stable isotope labeled (SIL, or “heavy labeled”) full length proteins designed to be used as mass spectrometry internal standards for quantitative proteomics. We also offer all the SILu™Prot products in their unlabeled form, SILu™Lite proteins. Additionally, our SILu™MAb and SILu™Lite Antibody Standards are recombinant IgG1 monoclonal antibody standards that are useful to assess the pharmacokinetic properties of biotherapeutics, such as monoclonal therapeutic antibodies and Fc-fusion therapeutics. SILu™MAb is a stable isotope labeled antibody, while SILuLite is the corresponding unlabeled form of the antibody.
SCIEX iChemistry™ Solutions
In collaboration with SCIEX, we now provide their global distribution mass spectrometry-based tagging chemistries, SCIEX iChemistryTM Solutions, for use in basic research and applied markets. The two companies together provide total reagent solutions for quantitative proteomics, to allow researchers to dig deeper into complex biological systems, analyze prospective biomarkers, and deliver results. You now have access to the largest selection of high-quality tools for workflows to enable biomarker analysis. Specifically, in protein biology, our offerings include the SCIEX ICAT®, iTRAQ®, mTRAQ®, and CYP450 product lines for quantitative proteomics. With our targeted selection of kits, tagging reagents, calibrants, and MS standards, we are committed to meet your mass spectrometry reagent needs.
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