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Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli
In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either
MISSION shRNA Lentiviral Transduction
MISSION® shRNA Lentiviral Transduction application data
Targeted Genome Editing Using Engineered Zinc Finger Nucleases
Targeted genome editing with Zinc finger nucleases (ZFNs) has a wide variety of applications, and three key modes of action have been a focus thus far: targeted gene knockouts, targeted gene integration, and targeted gene correction.
Webinar: SygRNA®-Synthetic Two Part CRISPR RNA System
The CRISPR/Cas genome editing system has revolutionized most every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA, or lentivirus.
Lentivirus Cell Line
Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.
CRISPR-based Gene Activation
Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.
CRISPR/CAS9 Gene Editing Protocol for Human Induced Pluripotent Stem Cells (iPSCs)
This article provides an extensive step-wise overview of CRISPR Cas9 protocol that can be used to perform gene editing in human induced pluripotent stem cells (iPSCs).
MVM Resistance Through Genetic Engineering
As part of the CHOZN brand promise to enable the development of better biologics and safer manufacturing processes, we have identified a gene target (SLC35A1) that upon elimination of expression by gene editing, results in a CHO cell line that
Genome Engineering with CompoZr Custom Zinc Finger Nucleases (ZFNs)
Genome Editing is a powerful technique that can be used to elucidate gene function and the genetic basis of disease. Watch our video.
Bacterial Transformation
Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.
Advanced RNAi Applications — in vivo FAQs
We answer your frequently asked questions about in vivo RNAi applications.
How Do ZFNs Work?
CompoZr Zinc Finger Nucleases (ZFNs) are used to create modified cell lines with targeted gene deletions, gene insertions, or gene corrections.
CRISPR on the Brain? Standardized Functional Genomics Tools Advance Neuroscience
Deciphering the genetics of neuroscience has always been challenging. Heterogeneous tissue microenvironments, complex genetic interactions and phenotypes, lack of model systems that accurately mimic the human brain transcriptome - not to mention the blood-brain barrier - make genetic perturbation analysis
NitroGENIUS: Engineering E. coli to Fix Nitrogen and Regulating Transcription with Light
Endosymbiotic theory states that chloroplasts in plants originally came from a symbiotic relationship between prokaryotes.
CRISPR Essentials: MISSION® CRISPR gRNAs, Cas9 and Related Products
Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments.
Successful Transduction Using Lentivirus
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
Nuclease-Free Water at Your Fingertips
An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.
Precision Genetic Modifications
Our scientists’ years of experience and Sigma-Aldrich’s access to multiple advanced gene editing technologies allow us to offer a cell engineering service with unparalleled success. We can engineer a variety of modifications in your cell line of choice to address
Small RNA and miRNA (microRNA) Sequencing
A review on reducing miRNA and other small RNAs sequencing bias with RealSeq®-AC RNA library preparation on the Illumina® RNA sequencing platform.
Zinc Finger Nuclease References
Zinc Finger Nuclease References: Human, Rat, Mouse, Zebrafish, Plasmodium, Xenopus, Rainbow Trout, Mosquito, Pig, Rabbit, Cattle, CHO, ZFN Technology, Reviews, Posters, Label License
Small Molecule CRISPR Enhancers
The CRISPR-Cas9 system is an RNA-guided genome-editing tool that provides researchers a simple, easy, and quick way to modify the genomes of various organisms.
ZFN FAQs
Zinc Finger Nuclease - frequently asked questions
CRISPR Engineered MDCKII Cells Without Canine P-glycoprotein
Madin-Darby Canine Kidney (MDCK) cells are one of the most widely used cell lines for a variety of research applications, including drug permeability and transporter studies.
Viral Invaders
Protection at the genetic level – a new line of defense against contamination in biopharmaceutical manufacturing.
Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines
Our company presents an article on the Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines
TRC Design and pLKO.1 Vector FAQs
The MISSION® shRNA clones, designed by the TRC, are pre-cloned into the pLKO.1-Puro vector. This lentiviral vector allows for propagation in bacterial culture and selection of inserts in mammalian cells.
Prime Editing – An Exciting Combination of Natural and Engineered RNA-guided Genome Editing
Prime Editing is a novel variation on CRISPR systems which expands the guide RNA’s responsibility to serve two purposes: (1) to guide Cas9 to a targeted genomic location, and (2) to serve as an RNA template to copy new sequences
Reverse Transfection of Plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
Long Oligos
Our experience with gene construction and microarray development provides us with insight into the potential difficulties of long oligo synthesis. We have developed techniques to purify long oligos, which are unmatched by other suppliers.
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