Large molecule HPLC

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When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.

Ascentis® Express Phenyl Hexyl Columns for U/HPLC

Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.

BIOshell™ Glycan U/HPLC Columns

The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.

HPLC Columns for Antibody and Large Protein Analysis

BIOshell™ IgG 1000 Å C4 columns are highly suited for the reversed phase separation of high molecular weight compounds such as monoclonal antibodies with molecular weight of 150 kDa.

Columns and Analytical Standards for Protein SEC

Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.

Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard

Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.

SupraSolv® GC Solvent Specifications

Our GC solvents have been specially developed and tested for ECD, FID and MS detection or for headspace gas chromatography to ensure accurate, reliable and reproducible analytical results.

How to Select the Right Water Purification System for Your Lab

Technical article on Selecting the Right Water Purification System

Ascentis® Express Biphenyl UHPLC and HPLC Columns

The bonded, densely endcapped, dimethyl-biphenyl stationary phase of Ascentis® Express 90 Å Biphenyl, based on fused-core particles, provides a stable, reversed phase packing with enhanced π-π and mild steric interactions due to the two sequential phenyl groups bonded to the

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

Peptide Sample Amount Determination

Determining the amount of desired product in a peptide sample.

HPLC Tips & Tricks: Increase Your HPLC/UHPLC Method Sensitivity

Tips & tricks to improve your chromatographic method sensitivity by reducing baseline noise and/or making suggested improvements to increase signal intensity.

Chromatography Profile Analysis of Phosphorothioate Oligonucleotides

Understand the impact of phosphorothioate internucleotide linkage addition to oligonucleotide chromatography profiles.

HPLC Tips & Tricks: Optimizing Injection Volume

Learn the tips and tricks to optimize the injection volume during your High-Performance Liquid Chromatography (HPLC) method-based analyses and further, how it affects your peak area.

Fast and high-resolution Analysis of Intact Therapeutic Monoclonal Antibody Trastuzumab using BIOshell™ A400 Protein C4 Column

Optimization of a Reversed-Phase Liquid Chromatographic (RP-LC) method for the intact analysis of a therapeutic monoclonal antibody, trastuzumab, using a BIOshell™ A400 Protein C4 column.

Supelco Preparative HPLC products for Pharmaceutical Development and Production

HPLC Tips & Tricks – Mobile Phase Preparation

Tips and tricks to prepare the correct and consistent mobile phase for optimized separations during your High-performance Liquid Chromatographic (HPLC) based method developments and optimizations.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

New Certified Reference Materials for Proteins

CRMs of milk proteins aid accurate testing for infant formula, ensuring compliance and quality control.

Factors Affecting Resolution in HPLC

Review the effects of efficiency (plate number), retention, and selectivity factors on HPLC resolution. See how factors relate to particle and column characteristics via the HPLC resolution equation.

Ascentis® Express RP-Amide HPLC Columns

Innovative particle technology and RP-Amide bonding chemistry enhance chromatography performance for improved peak shape and resolution.

Quality Attribute Determination of Monoclonal Antibodies

Workflows for monoclonal antibody adalimumab characterization ensure drug safety and efficacy through critical quality attribute analysis.

Difluoroacetic Acid as an Effective Mobile Phase Modifier for the LC-UV/MS Analysis of Proteins

The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.

How to Maximize Sensitivity in LC-MS

LC-MS sensitivity optimization tips minimize contaminations, enhancing detection limits and spectrum clarity for accurate analysis.

Analytical SEC Columns for Biomolecule Separations

Size exclusion chromatography columns enhance biomolecule separation efficiency with sub-2 μm particles, improving analytical performance.

Cannabinoids Analysis Workflow with Chromolith® Column

HPLC-DAD workflow analyzes 14 cannabinoids in hemp extracts in 10 minutes using Chromolith columns for robust performance.

An LC/MS Peptide Standard for Rapid System Suitability Assessment and Retention Time Translation Across LC/MS Platforms

Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis.

Ephedrine/Pseudoephedrine HCl in Oral Solution Analysis

HPLC method on Purospher® RP-18e column analyzes ephedrine and pseudoephedrine HCl in traditional Chinese medicine.

Antibody Aggregates & Fragments Analysis Workflow

Characterize mAb monomers, aggregates, and fragments using SEC-UV workflow with Zenix® and Zenix®-C SEC columns.

UHPLC Vitamin D Analysis on Supel™ Carbon LC Columns

UHPLC separation of Vitamin D Metabolites using Supel™ Carbon LC column ensures excellent peak shape and sensitivity.

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