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Molecular Beacons
Molecular Beacons are structured probes that are highly sensitive, sequence specific, and are used for sequence detection in qPCR and in vitro studies.
Whole Genome Amplification for Single Cell Biology
The ultimate biological unit lies within a single cell. Many biological disciplines have taken aim to elucidate the causes of cellular differentiation at this level.
PCR Troubleshooting Tips
When developing a PCR troubleshooting protocol, it is important to be open to any possible sources of error, however insignificant they may seem, in order to explore each potential problem independently.
Oligonucleotide Quality Control by Mass Spectrometry
Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.
OligoArchitect™ Assay Design
Efficient qPCR relies on good assay design. Since the invention of PCR, many parameters have been identified as important for assay quality, such as estimates of oligo temperature characteristics, GC content and folding properties.
Designing Peptides
While peptide synthesis can be straightforward, it is important to consider several factors before synthesis commences. The sequence, amino acid composition and length of a peptide will influence whether correct assembly and purification are feasible.
Extract-N-Amp™ Tissue Feature Article
Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.
GenomePlex® Whole Genome Amplification Kit: A High-Throughput Approach for Rapidly Amplifying Genomic DNA from a Variety of Biological Materials
GenomePlex® Whole Genome Amplification Kit: A High-Throughput Approach for Rapidly Amplifying Genomic DNA from a Variety of Biological Materials
Polymerase Chain Reaction
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®
Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.
Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards
Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA
What is Hot Start PCR?
Learn about the benefits of using Hot Start PCR reagents to suppress enzymatic activity before PCR reaction tubes enter the thermal cycler.
PCR/qPCR/dPCR Assay Design
The entire PCR workflow is vulnerable to factors which introduce variability. Many of the variable components are unavoidable, such as the source of the sample or the requirement for a reverse transcription step. Assay design is also highly variable and
Colony PCR
Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.
Troubleshooting for Molecular Cloning
Molecular cloning is the process of inserting the gene-of-interest (GOI) into a plasmid vector and this vector is then inserted into a cell that expresses the protein encoded by the GOI. Once protein is expressed in the cell, the protein
Whole Transcriptome Amplification of RNA from Low Cell-Number Samples
The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.
Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method
In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, somewhat hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome
ThermaStop™ PCR Additive
PCR additive added to master mix that stops nonspecific bands after cool down.
GC-RICH PCR System Troubleshooting
GC-RICH Amplification of Polymerase Chain Reaction (PCR) System Troubleshooting.
5'/3' RACE Kit, 2nd Generation Troubleshooting
In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction
Mycoplasma PCR ELISA Protocol
Mycoplasmas are potential contaminants in mammalian cell culture manufacturing. All products produced in cell culture to be tested for the presence of Mycoplasma
Troubleshooting PCR and RT-PCR Amplification
This page shows PCR and RT-PCR amplification troubleshooting.
Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)
Preserved samples from medical, forensic, museum and other archival collections represent a rich source of study material, much of it meticulously collected, characterized and preserved through many decades of work by experts in the field.
Whole Genome Amplification for Single Cell Biology
Whole genome amplification (WGA) offers a means to overcome the above restrictions for single-cell genomic analyses. WGA has been described as a non-specific amplification technique that affords an amplified product completely representative of the initial starting material.
Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA
The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis
Quantitative PCR and Digital PCR Detection Methods
Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.
Retinoic Acid and Gene Expression
All-trans retinoic acid (RA, ATRA) is a pleiotropic activation factor that regulates genes associated with normal vertebrate cellular processes such as cell differentiation, cell proliferation, apoptosis, and embryonic development.
Extract-N-Amp™ Plant Tissue PCR Kits
The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that
Nuclease-Free Water at Your Fingertips
An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.
Dual-Labeled Probes in Digital PCR
Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.
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