Protein and nucleic acid interactions

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Duolink® PLA Multicolor Detection Protocol

Guide to create custom PLA probes for immunofluorescent or brightfield detection.

Purification or Removal of DNA-Binding Proteins

This page shows how to purify or remove DNA-binding proteins with Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin from Cytiva.

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

Using Duolink® in Protein Interaction Studies

The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.

RNA Immunoprecipitation qPCR (RIP- qPCR) Protocol

RNA immunoprecipitation (RIP) can identify specific RNA molecules of many types associated with specific nuclear or cytoplasmic binding proteins.

Chromatin Isolation by RNA Purification (ChIRP) Protocol

Chromatin Isolation by RNA purification (ChIRP) protocol for isolating RNA-bound genome regions.

Creating PLA® Probes

Duolink® kits use in situ PLA® technology for accurate quantification of individual proteins, interactions, and modifications in cells and tissue.

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

Counterstaining after the Duolink In Situ Protocol

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

Synthetic Peptide Handling & Storage Protocol

Solvent choice is crucial for synthetic peptide handling, with guidelines provided for proper dissolution.

Duolink® PLA Brightfield Protocol

Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.

Using Duolink™ in Multiwell Plates

Duolink protocol modifications for staining cells in multiwell plates; adapt washing, imaging, and scanning for multiwell format.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.