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  • Single-step PCR-based genetic sex determination of rat tissues and cells.

Single-step PCR-based genetic sex determination of rat tissues and cells.

BioTechniques (2017-05-23)
Pramod Dhakal, Michael J Soares
ABSTRACT

The advent of genome editing strategies has expanded the range of animal models available for gene manipulation and renewed research interest in the rat. Gender is a key variable for in vivo gene function analyses. Here, we present a simple PCR-based method to determine genetic sex in the rat.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Extract-N-Amp Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications