- Rapid diagnosis of phenylketonuria and other aminoacidemias by quantitative analysis of amino acids in neonatal blood spots by gas chromatography-mass spectrometry.
Rapid diagnosis of phenylketonuria and other aminoacidemias by quantitative analysis of amino acids in neonatal blood spots by gas chromatography-mass spectrometry.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2002-07-09)
Chunhui Deng, Chunqing Shang, Yaoming Hu, Xiangmin Zhang
PMID12101068
ABSTRACT
A new method for quantifying specific amino acids in small volumes of plasma and whole blood has been developed. Volatile derivatives of amino acids are analyzed by gas chromatography-mass spectrometry. The method only takes a few minutes to perform and requires minimal sample preparation. The accurate assay of phenylalanine, tyrosine and other amino acids in dried blood spots could be used for neonatal screening for phenylketonuria and other aminoacidemias. Because of the low cost, this neonatal screening method is suited to application in developing countries such as China.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
L-Isoleucine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
L-Phenylalanine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Supelco
L-Isoleucine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Supelco
L-Phenylalanine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Supelco
17α-Hydroxyprogesterone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®