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The oxysterol and cholestenoic acid profile of mouse cerebrospinal fluid.

The oxysterol and cholestenoic acid profile of mouse cerebrospinal fluid.

Steroids (2015-03-12)

Peter J Crick, Lien Beckers, Myriam Baes, Paul P Van Veldhoven, Yuqin Wang, William J Griffiths

ABSTRACT

Oxysterols and cholestenoic acids are oxidised forms of cholesterol with a host of biological functions. The possible roles of oxysterols in various neurological diseases makes the analysis of these metabolites in the central nervous system of particular interest. Here, we report the identification and quantification of a panel of twelve sterols in mouse cerebrospinal fluid (CSF) using liquid chromatography-mass spectrometry exploiting enzyme assisted derivatisation for sterol analysis technology. We found low levels of oxysterols and cholestenoic acids in CSF in the range of 5pg/mL-2.6ng/mL. As found in man, these concentrations are one to two orders of magnitude lower than in plasma.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Cholesterol, powder, BioReagent, suitable for cell culture, ≥99%

Sigma-Aldrich

Cholesterol, Sigma Grade, ≥99%

Sigma-Aldrich

Cholesterol, from sheep wool, ≥92.5% (GC), powder

Sigma-Aldrich

Isopropyl alcohol, ≥99.7%, FCC, FG

Sigma-Aldrich

Acetic acid, natural, ≥99.5%, FG

Sigma-Aldrich

Acetic acid, ≥99.5%, FCC, FG

Sigma-Aldrich

Acetic acid-¹²C₂, 99.9 atom % ¹²C

Sigma-Aldrich

SyntheChol^® NS0 Supplement, 500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture

Sigma-Aldrich

Methanol, suitable for NMR (reference standard)

Sigma-Aldrich

Acetic acid, suitable for luminescence, BioUltra, ≥99.5% (GC)

SAFC

Cholesterol, from sheep wool, Controlled origin, meets USP/NF testing specifications

Sigma-Aldrich

Acetonitrile, anhydrous, 99.8%

Sigma-Aldrich

Formic acid, ≥95%, FCC, FG

Sigma-Aldrich

5α-Androstan-17β-ol-3-one, purum, ≥99.0% (TLC)

Sigma-Aldrich

Potassium phosphate monobasic, BioUltra, Molecular Biology, anhydrous, ≥99.5% (T)

Sigma-Aldrich

2-Propanol, BioUltra, Molecular Biology, ≥99.5% (GC)

Sigma-Aldrich

5α-Androstan-17β-ol-3-one, ≥97.5%

Sigma-Aldrich

2-Propanol, BioReagent, ≥99.5%, Molecular Biology

Sigma-Aldrich

Potassium phosphate monobasic, ReagentPlus^®

Sigma-Aldrich

Potassium phosphate monobasic, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

Sigma-Aldrich

Potassium phosphate monobasic, Molecular Biology, ≥98.0%

Sigma-Aldrich

2-Propanol, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%, poly coated bottles

Sigma-Aldrich

Potassium phosphate monobasic, 99.99% trace metals basis

Sigma-Aldrich

Ultrapure Acetonitrile

Sigma-Aldrich

2-Propanol, electronic grade, 99.999% trace metals basis

Sigma-Aldrich

Acetonitrile, electronic grade, 99.999% trace metals basis

Avanti

7α-Hydroxy-3-oxocholest-4-enoic acid-d3, Avanti Research^™ - A Croda Brand

Avanti

3β,7α-Dihydroxycholest-5-enoic acid-d3, powder, Avanti Research^™ - A Croda Brand