Through their mix-and-match ability, ColorWheel® flow cytometry antibodies and dyes are designed to provide greater flexibility in multicolor assay design and expansion than pre-conjugated primary antibodies, while offering enhanced simplicity compared to secondary conjugated antibodies and labeling kits. See just how simple it is to create your own optimal flow cytometry reagents with our 3-Step ColorWheel® Flow Cytometry Reagent Preparation protocol.
To learn more about using ColorWheel® antibodies and dyes, see our Frequently Asked Questions page.
This protocol requires the following materials:
You will need one ColorWheel® Antibody-Ready Dye for each ColorWheel® Dye-Ready Antibody you plan to label for this protocol along with 75 µL of PBS. The ColorWheel® finishing buffer is packaged along with each ColorWheel® Antibody-Ready Dye.
The ColorWheel® Flow Cytometry Reagent Preparation protocol takes less than 5 minutes of hands-on time for a simplified reagent preparation for flow cytometry experiments.
There are just 3 steps to create your optimal reagents:
Figure 1 shows step 1 of the ColorWheel® protocol, which is:
Figure 1.Step 1 in the ColorWheel® Flow Cytometry Reagent Preparation protocol. Spin down the ColorWheel® dye, ColorWheel® antibody, and ColorWheel® finishing buffer and then resuspend.
Figure 2.Step 2 in the ColorWheel® Flow Cytometry Reagent Preparation protocol. Mix the ColorWheel® antibody and dye and incubate at 27 °C/room temperature for 30 minutes.
Figure 3 shows step 3 of the ColorWheel® protocol, which is:
To see how the completed reagent fits into the overall flow cytometry workflow, see our Flow Cytometry Guide.
Figure 3.Step 3 in the ColorWheel® Flow Cytometry Reagent Preparation protocol. Add ColorWheel® finishing buffer to the ColorWheel® antibody/dye mixture and incubate at 27 °C/room temperature for 30 minutes. The solution is ready to add to the sample.
Discover tips on finding the right fluorochrome for your experiment with our guides on selecting the right fluorochrome for your flow cytometry experiment and building the optimal panel for multicolor analysis or browse our troubleshooting guide for further advice.
Get started on creating your optimal reagents with ColorWheel® flow cytometry antibodies and dyes. You can also request more information about our trial offer for a discount on your first order.