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HomeFlow CytometryFlow Cytometry Troubleshooting Guide: How to Improve Flow Cytometry Analysis

Flow Cytometry Troubleshooting Guide: How to Improve Flow Cytometry Analysis

Flow cytometry characterizes and/or sorts heterogeneous suspended cell populations based on physical and fluorescence characteristics. For optimum results and data analysis, a basic understanding of fluorochromes and their characteristics, spectral overlap and spillover, compensation and spread, as well as flow cytometer variables that can affect the sensitivity of the measurements is necessary.

The following flow cytometry troubleshooting guide describes possible causes and solutions for most common problems encountered during flow cytometry experiments. Click on the common problems below for tips on how to improve your flow cytometry analysis, organized in easy-to-use tables.

We are committed to providing the best solutions and services to researchers around the globe. This flow cytometry troubleshooting guide does cover most commonly encountered problems. However, some problems may be related to one’s specific experiment. Please contact our Technical Service team for any specific issue you may encounter with your flow cytometric application of our antibodies.

Read How to Select Flow Cytometry Dyes for more tips to enhance your flow cytometry experiments.

Acquisition Rate Decreases Dramatically

A Loss or Lack of Signal

High Background and/or Non-Specific Staining of Cells

Variability In Results From Day to Day

High Fluorescent Intensity

Suboptimal Cell Scatter Properties

Antibody Worked In Other Applications But Not In Flow Cytometry