Merck
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230162

Sigma-Aldrich

Sulforhodamine B

Dye content 75 %

Synonym(s):
Acid Red 52, Sulforhodamine B monosodium salt
Empirical Formula (Hill Notation):
C27H29N2NaO7S2
CAS Number:
Molecular Weight:
580.65
Numer w indeksie barw:
45100
Beilstein:
3886008
Numer EC:
Numer MDL:
Identyfikator substancji w PubChem:
NACRES:
NA.47

forma

powder

Poziom jakości

100

skład

Dye content, 75%

rozpuszczalność

methanol: 1 mg/mL

λmax

554 nm

ε (extinction coefficient)

≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

temp. przechowywania

room temp

SMILES string

[Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1

InChI

1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1

InChI key

SXQCTESRRZBPHJ-UHFFFAOYSA-M

Related Categories

Opis ogólny

Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.

Zastosowanie

Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.

Opakowanie

1, 5 g in glass bottle

Kod klasy składowania

13 - Non Combustible Solids

WGK

WGK 2

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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