Wszystkie zdjęcia(2)

AB1726

Sigma-Aldrich

Anti-Connexin 40 Antibody

Chemicon®, from rabbit

Synonim(y):
Cx40, Gap Junction alpha-5 Protein, CxA-5
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

100

forma przeciwciała

affinity isolated antibody

antibody product type

primary antibodies

klon

polyclonal

oczyszczone przez

affinity chromatography

species reactivity

rat, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

numer dostępu NCBI

numer dostępu UniProt

transport

wet ice

Gene Information

human ... GJA5(2702)

Opis ogólny

Mouse Connexin 40 is a 358 amino acid gap junction protein with a predicted M.W. of ~40 kDa. It is prominently expressed in lung, heart and skin (see reviews: Kumar & Giula 1996; White, 1995; Evans, 1994; Beyer , 1990; Henneman, 1992; Traub, 1994; Goliger & Paul 1994). One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins.

Specyficzność

Reacts with mouse connexin 40.
Reactivity with other species has not been determined.

Immunogen

Anti-Connexin 40 is made against a 19 amino acid peptide sequence within the C-terminal cytoplasmic domain of mouse C×40 (2). Peptide sequence is conserved in human and rat Cx40 (100%), chicken Cx42 (77%), human Cx50 (87%), rat Cx50 (81%) and sheep Cx49 (87%).

Zastosowanie

Anti-Connexin 40 Antibody is an antibody against Cx40 for use in IHC & WB.
Immunohistochemistry Analysis: An 1:50 dilution of a representative lot detected Connexin 40 immunoreactivity associated with the gap junction between the cardiac myoepithial cells in mouse heart tissue sections.
Western Blot Analysis: An 1:1000 dilution of a representative lot detected Connexin 40 in human (HEK293, HeLa, HepG2, HUVEC, Jurkat) and murine (C2C12, HIN/3T3) cell lysates.
Western Blot Analysis: An 1:000 dilution of a representative lot detected Connexin 40 in human, mouse, and rat heart tissue homogenates, as well as in mouse brain tissue homogenates.

Optimal working dilutions must be determined by end user.

Jakość

Evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis: A 1:1000 dilution of this lot detected Connexin 40 in 10 μg of Jurkat lysates.

Opis wartości docelowych

40 kDa

Postać fizyczna

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 15 mM NaCl containing 0.05% sodium azide.

Komentarz do analizy

Control
Placenta lysate.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Kod klasy składowania

12 - Non Combustible Liquids

WGK

WGK 1

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certyfikat analizy

Świadectwo pochodzenia

Volker J Schmidt et al.
PloS one, 8(11), e78782-e78782 (2013-11-16)
Gap junctions are involved in vascular growth and their expression pattern is modulated in response to hemodynamic conditions. They are clusters of intercellular channels formed by connexins (Cx) of which four subtypes are expressed in the cardiovascular system, namely Cx37
Connexin expression and conducted vasodilation along arteriolar endothelium in mouse skeletal muscle.
Looft-Wilson, Robin C, et al.
Journal of Applied Physiology, 97, 1152-1158 (2004)
Independence of connexin expression and vasomotor conduction from sympathetic innervation in hamster feed arteries.
Robin C Looft-Wilson, Sara J Haug, P Darrell Neufer, Steven S Segal
Microcirculation (New York, N.Y. : 1994) (1994)
GM-CSF causes a Paradoxical Increase in the BH3-only Pro-Apoptotic Protien BIM in Human Neutrophils.
Cowburn AS, Summers C, Dunmore BJ, Farahi N, Hayhoe RP, Print CG, Cook SJ, Chilvers ER
American Journal of Respiratory Cell and Molecular Biology null
Role of connexin40 in the autoregulatory response of the afferent arteriole.
Sorensen, CM; Giese, I; Braunstein, TH; Brasen, JC; Salomonsson, M; Holstein-Rathlou, NH
American Journal of Physiology: Renal Physiology null

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