Merck
All Photos(2)

MABN476

Sigma-Aldrich

Anti-Paris/ZNF746 Antibody, clone N196/16

clone N196/16, from mouse

Synonym(s):
Zinc finger protein 746, Parkin-interacting substrate, PARIS
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

100

forma przeciwciała

purified immunoglobulin

antibody product type

primary antibodies

klon

N196/16, monoclonal

species reactivity

human, rat, mouse

technique(s)

western blot: suitable

izotyp

IgG1κ

numer dostępu NCBI

numer dostępu UniProt

transport

wet ice

Gene Information

human ... ZNF746(155061)

Opis ogólny

Zinc finger protein 746 (ZNF746) is also called Parkin-interacting substrate (PARIS). ZNF746 is a repressor of transcription that binds to promoters and represses the transcription of PGC-1-alpha (PPARGC1A), making it important to the regulation of neuron death. ZNF746 may also contribute to neurodegeneration in Parkinson disease.

Specyficzność

This antibody recognizes the N-terminus of ZNF746. This antibody does not cross react with ZNF783 or ZNF398 (Prof. J. Trimmer, University of California, Davis.).

Immunogen

Epitope: N-terminus
Recombinant protein corresponding to the N-terminus of human Paris/ZNF746.

Zastosowanie

Anti-Paris/ZNF746 Antibody, clone N196/16 is a highly specific mouse monoclonal antibody & that targets Zinc Finger Protein & has been tested in western blotting.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western Blotting Analysis: 1 µg/mL of this antibody detected Paris/ZNF746 in 10 µg of RAW264.7 cell lysate. Immunohistochemistry Analysis: A representative lot detected Paris/ZNF746 in rat brain tissue (Prof. J. Trimmer, University of California, Davis.).

Jakość

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Paris/ZNF746 in 10 µg of HEK293 cell lysate.

Opis wartości docelowych

~72 kDa observed. Uniprot describes 3 isoforms, two at 69 kDa and one at 49 kDa This protein is ubiquitinated and has been observed at higher molecular weights (Shin, J. H., et al. (2011). Cell. 144(5):689-702.).

Postać fizyczna

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
HEK293 cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Kod klasy składowania

12 - Non Combustible Liquids

WGK

WGK 1

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service