MABN504

Sigma-Aldrich

Anti-VDAC1 Antibody, clone N152B/23

clone N152B/23, from mouse

Synonim(y):
Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM
eCl@ss:
32160702
NACRES:
NA.41

Poziom jakości

100

pochodzenie biologiczne

mouse

forma przeciwciała

purified antibody

antibody product type

primary antibodies

klon

N152B/23, monoclonal

species reactivity

mouse, rat, human

opakowanie

antibody small pack of 25 μg

zastosowania

immunohistochemistry: suitable
western blot: suitable

izotyp

IgG2aκ

numer dostępu NCBI

numer dostępu UniProt

wysyłka w ciągu

ambient

temp. przechowywania

2-8°C

Gene Information

human ... VDAC1(7416)

Powiązane kategorie

Opis ogólny

Voltage-dependent anion-selective channel protein 1 (VDAC1, VDAC), also known as outer mitochondrial membrane protein porin 1, is the outer mitochondrial membrane receptor for hexokinase and BCL2L1. VDAC1 forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation and apoptosis. VDAC1 may participate in the formation of the permeability transition pore complex (PTPC), which is responsible for the release of mitochondrial products that triggers apoptosis.

Specyficzność

This antibody does not cross-react with VDAC2 or VDAC3 (Prof. J. Trimmer, University of California, Davis).

Immunogen

Recombinant protein corresponding to human VDAC1.

Zastosowanie

Research Category
Neuroscience
Western Blotting Analysis: A representative lot states VDAC1 does not cross-react with VDAC2 or VDAC3 (based on KO validation results) representative lot deteted VDAC1 in wild type mouse liver tissue, and demonstrated a loss of signal in VDAC1 knock out mouse hippocampal tissue using immunobloting analysis (Prof. J. Trimmer, University of California, Davis).
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected VDAC1 in human cardiac myocytes tissue.
Research Sub Category
Developmental Signaling
Detect VDAC using this mouse monoclonal antibody, Anti-VDAC1 Antibody, clone N152B/23 validated for use in western blotting & IHC.

Jakość

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected VDAC1 in 10 µg of mouse brain tissue lysate.

Opis wartości docelowych

~ 35 kDa observed

Postać fizyczna

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Mouse brain tissue lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certyfikat analizy

Świadectwo pochodzenia

Maria Feola et al.
Communications biology, 4(1), 517-517 (2021-05-05)
Erythropoiesis involves complex interrelated molecular signals influencing cell survival, differentiation, and enucleation. Diseases associated with ineffective erythropoiesis, such as β-thalassemias, exhibit erythroid expansion and defective enucleation. Clear mechanistic determinants of what make erythropoiesis effective are lacking. We previously demonstrated that...
Abdulhafiz D Imam Aliagan et al.
American journal of translational research, 12(11), 7542-7564 (2020-12-15)
Mitochondrial dysfunction plays a critical role in the pathophysiology of Parkinson's disease (PD). The inner mitochondrial membrane (IMM) protein, Mitofilin or Mic60, has been shown to play a key role in controlling and maintaining mitochondrial cristae morphology, and its dysregulation...
Gui-Ying Yao et al.
Cell death & disease, 9(10), 1033-1033 (2018-10-12)
Ischemic postconditioning provides robust neuroprotection, therefore, determining the molecular events may provide promising targets for stroke treatment. Here, we showed that the expression of functional mitochondrial voltage-dependent anion channel proteins (VDAC1, VDAC2, and VDAC3) reduced in rat vulnerable hippocampal CA1...
Arnaud Mourier et al.
The Journal of cell biology, 208(4), 429-442 (2015-02-18)
Mitochondria form a dynamic network within the cell as a result of balanced fusion and fission. Despite the established role of mitofusins (MFN1 and MFN2) in mitochondrial fusion, only MFN2 has been associated with metabolic and neurodegenerative diseases, which suggests...
Chong Kiat Goo et al.
PloS one, 7(9), e45806-e45806 (2012-10-11)
Akt, a serine/threonine kinase has been shown to stimulate glycolysis in cancer cells but its role in mitochondrial respiration is unknown. Using PTEN-knockout mouse embryonic fibroblasts (MEF(PTEN-/-)) with hyper-activated Akt as a cell model, we observed a higher respiratory capacity...

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