Przejdź do
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 2000 tests | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 4200,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352200
NACRES:
NA.54
4200,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócusage
sufficient for ≤2,000 tests
packaging
pkg of 1 kit
manufacturer/tradename
Roche
technique(s)
protein quantification: suitable
λmax
490 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
−20°C
General description
A nonradioactive alternative to the [51Cr] and [3H]-thymidine release assay.
Colorimetric assay for the quantification of cell death and cell lysis, based on the measurement of lactate dehydrogenase (LDH) activity released from the cytosol of damaged cells into the supernatant.
Colorimetric assay for the quantification of cell death and cell lysis, based on the measurement of lactate dehydrogenase (LDH) activity released from the cytosol of damaged cells into the supernatant.
Application
The Cytotoxicity Detection Kit (LDH) is a fast and simple method to quantify cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well plate format. Thus, the kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example:
- Detection and quantification of cell-mediated cytotoxicity
- Determination of mediator-induced cytolysis
- Determination of the cytotoxic potential of compounds in environmental and medical research, and in the food, cosmetic, and pharmaceutical industries[1][2][3]
- Determination of cell death in bioreactors
- Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth[4][5]
Packaging
1 kit containing 2 components.
Preparation Note
Working solution: Vial 1 Catalyst:
Reconstitute the lyophilisate in 1 ml double dist. water for 10 minutes and mix thoroughly.
Vial 2 Dye solution:
Ready-to-use solution.
Reaction mixture
For 100 tests: Shortly before use, mix 250 μl of vial 1 with 11.25 ml of vial 2.
For 400 tests: Shortly before use, add the total volume of catalyst (1 ml) to the total volume
of dye solution (45 ml) and mix well.
Note: The Reaction mixture should not be stored; prepare immediately before use.
Storage conditions (working solution): 2 to 8 °C
The lyophilizate is stable at 2 to 8 °C.
The reconstituted catalyst solution is stable for four weeks when stored at 2 to 8 °C.
Once thawed, the dye solution is stable for several weeks when stored at 2 to 8 °C.
Reconstitute the lyophilisate in 1 ml double dist. water for 10 minutes and mix thoroughly.
Vial 2 Dye solution:
Ready-to-use solution.
Reaction mixture
For 100 tests: Shortly before use, mix 250 μl of vial 1 with 11.25 ml of vial 2.
For 400 tests: Shortly before use, add the total volume of catalyst (1 ml) to the total volume
of dye solution (45 ml) and mix well.
Note: The Reaction mixture should not be stored; prepare immediately before use.
Storage conditions (working solution): 2 to 8 °C
The lyophilizate is stable at 2 to 8 °C.
The reconstituted catalyst solution is stable for four weeks when stored at 2 to 8 °C.
Once thawed, the dye solution is stable for several weeks when stored at 2 to 8 °C.
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| technique(s) protein quantification: suitable | technique(s) protein quantification: suitable | technique(s) protein quantification: suitable | technique(s) protein quantification: suitable |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename - |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric, fluorometric |
| application(s) cell analysis | application(s) cell analysis | application(s) cell analysis | application(s) cell analysis |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| packaging pkg of 1 kit | packaging kit of 1 (4 components) | packaging pkg of 1 kit | packaging pkg of 1 kit |
Tylko elementy zestawu
Numer produktu
Opis
- Catalyst (Diaphorase/NAD+ mixture)
- Dye Solution (INT and sodium lactate)
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Produkty
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
Powiązane treści
Zestaw do wykrywania cytotoksyczności (LDH) Protokół i rozwiązywanie problemów
Cytotoxicity Detection Kit (LDH) Protocol & Troubleshooting
Sabine Sewing et al.
PloS one, 11(7), e0159431-e0159431 (2016-07-22)
Single stranded oligonucleotides (SSO) represent a novel therapeutic modality that opens new space to address previously undruggable targets. In spite of their proven efficacy, the development of promising SSO drug candidates has been limited by reported cases of SSO-associated hepatotoxicity.
Guo-qian He et al.
Molecular brain, 8, 88-88 (2015-12-25)
Growth arrest and DNA-damage inducible protein 45 beta (Gadd45b) is serving as a neuronal activity sensor. Brain ischemia induces the expression of Gadd45b, which stimulates recovery after stroke and may play a protective role in cerebral ischemia. However, little is
Volker Gassling et al.
Clinical oral implants research, 24(3), 320-328 (2011-11-19)
The loss of jaw bone caused by different kinds of pathologies leads to dysfunction and reduced quality of life in affected patients. Thus, the pivotal goal in bone tissue engineering is to reconstruct these defects. The essential precondition for new
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| 11644793001 | 04061838259035 |