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11684795910

Roche

In Situ Cell Death Detection Kit, Fluorescein

sufficient for ≤50 tests, suitable for detection

Synonim(y):
Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate), Fluorescein-12-UTP tetralithium salt

zastosowanie

sufficient for ≤50 tests

Poziom jakości

100

manufacturer/tradename

Roche

application(s)

detection

transport

dry ice

temp. przechowywania

−20°C

Powiązane kategorie

Opis ogólny

Kit for the detection and quantification of apoptosis at the single-cell level, based on labeling of DNA strand breaks (TUNEL technology); analysis by fluorescence microscopy or flow cytometry.
Widely used methods to determine apoptosis include the analysis of the genomic DNA by agarose-gel electrophoresis and DNA fragmentation assays based on 3H-thymidine and, alternatively, 5-Bromo-2′-deoxy-uridine. The methods involve the separation of fragmented, low molecular weight DNA from unfragmented, high molecular weight DNA in a given cell population. Thus, these methods do not provide information about the fate of an individual cell in a given cell population, or particularly, in tissue sections. Alternatively, individual apoptotic cells may be microscopically recognized because of the characteristic appearance of nuclear chromatin condensation and fragmentation, but this method is subjective and limited to a relatively narrow time window when the morphological changes are at a maximum.
The hallmark of apoptosis is DNA degradation, which in early stages, is selective to the internucleosomal DNA linker regions. The DNA cleavage may yield double-stranded and single-stranded DNA breaks (nicks). Both types of breaks can be detected by labeling the free 3′-OH termini with modified nucleotides (e.g., biotin-dUTP, DIG-dUTP, fluorescein-dUTP) in an enzymatic reaction. The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes the template-independent polymerization of deoxyribonucleotides to the 3′-end of single- and double-stranded DNA. This method has also been termed TUNEL (TdT-mediated dUTP-X nick end labeling). Alternatively, free 3′-OH groups may be labeled using DNA polymerases by the template-dependent mechanism called nick translation. However, the TUNEL method is considered to be more sensitive and faster.

Specyficzność

The TUNEL reaction preferentially labels DNA strand breaks generated during apoptosis. This allows discrimination of apoptosis from necrosis and from primary DNA strand breaks induced by cytostatic drugs or irradiation.

Zastosowanie

The In Situ Cell Death Detection Kit, Fluorescein,  is a precise, fast, and simple nonradioactive technique to detect and quantify apoptotic cell death at the single-cell level by fluorescence microscopy and quantitative detection by flow cytometry in cells and tissues. Thus, the In Situ Cell Death Detection Kit can be used in many different assay systems.
Examples are:
  • Detection of individual apoptotic cells in frozen and formalin-fixed tissue sections in basic research
  • Determination of sensitivity of malignant cells to drug-induced apoptosis in cancer research
  • Typing of cells undergoing cell death in heterogeneous populations by double staining procedures

Cechy i korzyści

  • Sensitive: The direct labeling procedure using fluorescein-dUTP reduces background labeling
  • Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction
  • Convenient: No secondary detection system required
  • Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis

Opakowanie

1 kit containing 2 components.

Uwaga dotycząca przygotowania

Working solution: Add total volume (50 μl) of Enzyme Solution to the remaining 450 μl Label Solution to obtain 500 μl TUNEL reaction mixture.
Mix well to equilibrate components.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

Tylko elementy zestawu

Numer produktu
Opis

  • Enzyme Solution (TdT)

  • Label Solution (fluorescein-dUTP)

piktogramy

Health hazardEnvironment

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Aquatic Chronic 2 - Carc. 1B Inhalation

Kod klasy składowania

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Temperatura zapłonu °F

does not flash

Temperatura zapłonu °C

does not flash

Certyfikat analizy

Wprowadź numer partii, aby wyszukać certyfikat analizy (COA).

Świadectwo pochodzenia

Wprowadź numer partii, aby wyszukać świadectwo pochodzenia (COO).

Quotes and Ordering

Zengxia Li et al.
Biologics : targets & therapy, 1(4), 455-463 (2007-12-01)
Medullary thyroid carcinoma (MTC), a neuroendocrine tumor arising from the thyroid gland, is known to be poorly responsive to conventional chemotherapy. The root of Stemona tuberosa Lour, also called Bai Bu, is a commonly used traditional Chinese anti-tussive medicine. The
Laura Perin et al.
PloS one, 5(2), e9357-e9357 (2010-03-03)
Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kit(pos) stem
Shih Lung Cheng et al.
Respiratory research, 10, 115-115 (2009-11-26)
Although both animal and human studies suggested the association between placenta growth factor (PlGF) and chronic obstructive pulmonary disease (COPD), especially lung emphysema, the role of PlGF in the pathogenesis of emphysema remains to be clarified. This study hypothesizes that
A García-Peiró et al.
International journal of andrology, 34(6 Pt 2), e546-e553 (2011-05-04)
This investigation was conducted to assess the baseline level of sperm DNA fragmentation (SDF) in a cohort of patients presenting chromosomal rearrangements (nine reciprocal translocations and two inversions). In a separate experiment, a dynamic analysis to calculate the rate of
Yanli Song et al.
American journal of physiology. Heart and circulatory physiology, 295(2), H677-H690 (2008-06-17)
Heterozygous bone morphogenetic protein receptor-II-knockout (BMPR2(+/-)) mice have a similar genetic trait like that in some idiopathic pulmonary arterial hypertension patients. To examine the effect of pulmonary endothelial injury in BMPR2(+/-) mice, we challenged the mice with two injections of

Produkty

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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