Quick Spin columns contain gel filtration matrices which allow large molecules (e.g., DNA or RNA) to pass through quickly while retaining small molecules (e.g., nucleotides). The Quick Spin format improves the molecular sieving concept by using centrifugation to separate DNA or RNA rapidly and cleanly from small contaminants.
Quick Spin columns are produced with material that is shown to have a high recovery of radiolabeled DNA ( ≥80%).
All tedious, time-consuming steps involved in preparing columns have already been performed. The columns are pre-packed and quality tested to ensure
- maximal retention of unincorporated nucleotides (≥95%), and
- absence of DNase contamination according to the current Quality Control procedures.
Pre-packed, pre-swollen, pre-spun columns.
Suspension of Sephadex G-25 in STE buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM NaCl).
Exclusion limit: 10-12 bp
Ready-to-use disposable column units designed to quickly and efficiently remove unincorporated precursors from DNA labeled by nick translation, end-labeling, polymerization reactions, and other labeling techniques.
Quick Spin columns are designed for use in low-speed, swinging-bucket centrifuges.
Komentarz do analizy
Sample size: up to 50 μl
Time required: 10 minuntes
For life science research only. Not for use in diagnostic procedures.