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Merck

63069

Sigma-Aldrich

Magnesium chloride solution

BioUltra, for molecular biology, ~1 M in H2O

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Synonim(y):
Magnesium Chloride hydrate, Magnesium chloride (MgCl2)hexahydrate, MgCl2
Wzór liniowy:
MgCl2
Numer CAS:
Masa cząsteczkowa:
95.21
Numer MDL:
Kod UNSPSC:
12352302
Identyfikator substancji w PubChem:
NACRES:
NA.26

klasa czystości

for molecular biology

Poziom jakości

linia produktu

BioUltra

Postać

liquid

stężenie

~1 M in H2O

metody

HPLC: suitable
flow cytometry: suitable

zanieczyszczenia

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.0002% total Nitrogen (N)

pH

5.0-7.5 (25 °C, 1 M in H2O)

rozpuszczalność

water: soluble

gęstość

1.08 g/mL at 20 °C

ślady anionów

phosphate (PO43-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

ślady kationów

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤10 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤50 mg/kg
Li: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤500 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

absorpcja UV

λ: 260 nm Amax: 0.05
λ: 280 nm Amax: 0.03

przydatność

suitable for molecular biology

ciąg SMILES

Cl[Mg]Cl

zastosowania

cell analysis
filtration
vaccine development

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

Klucz InChI

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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Opis ogólny

Magnesium chloride is widely used in polymerase chain reactions (PCR) buffers to supply magnesium ions. The concentration of Mg2+ is the most critical parameter that impacts both the efficiency and specificity of PCR when total genomic DNA is used, synthesis of long products, or during multiplex reactions. Elevated concentrations of Mg2+ enhance the stability of primer-template duplexes and the efficiency of amplification.

Zastosowanie

Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or other liver cells).
Magnesium chloride solution has been used in the reaction mix of reverse transcriptase enzyme, PFV RT. It has been used in the preparation of artificial cerebrospinal fluid (ACSF).

Inne uwagi

For additional information on our range of Biochemicals, please complete this form.

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves


Certyfikaty analizy (CoA)

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