S9430

SYBR^® Green I nucleic acid gel stain

10,000 × in DMSO

• Synonim(y): 728 zielony barwnik żelowy, Barwnik DNA, bezpieczniejsza bejca żelowa
• Numer CAS: 163795-75-3
• Numer MDL: MFCD00284715
• Kod UNSPSC: 12171500
• NACRES: NA.52

Właściwości

• Formularz: solution
• Poziom jakości: 200
• zastosowanie: 1.0 mL sufficient for 100 mini-gels
• charakterystyka ekologicznej alternatywy: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• stężenie: 10,000 × in DMSO
• metody: PCR: suitable
• kategoria ekologicznej alternatywy: Aligned,
• temp. przechowywania: −20°C

Opis

Opis ogólny

SYBR^® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system.[1] SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.[2]

Zastosowanie

SYBR^® Green I nucleic acid gel stain has been used for:

• the quantification of dsDNA[3]
• to stain DNA in polymerase chain reaction (PCR)[4]
• for comet assay technique[5]
• to assess spermatozoon membrane integrity[6]
• for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)[7]
• as a fluorescent dye in flow cytometry[8]
• real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA[8]

Cechy i korzyści

• An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
• It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
• It is less mutagenic than ethidium bromide in Ames tests
• It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
• Useful for many applications with a limited amount of DNA
• The binding of SYBR^® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
• Removal of this stain in-gel digestion and ligation techniques is not needed
• SYBR Green I is a greener alternative product to ethidium bromide for staining

Przechowywanie i stabilność

Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.

Informacje prawne

SYBR is a registered trademark of Life Technologies

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): 201.2 °F - closed cup
• Temperatura zapłonu (°C): 94 °C - closed cup
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)