Merck

S9430

Sigma-Aldrich

SYBR® Green I nucleic acid gel stain

greener alternative

10,000 × in DMSO

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Synonim(y):
DNA stain, SYBR® green gel dye, safer gel stain
Numer CAS:
Numer MDL:
NACRES:
NA.52

forma

solution

Poziom jakości

zastosowanie

 mL sufficient for 100 mini-gels

charakterystyka ekologicznej alternatywy

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

stężenie

10,000 × in DMSO

technique(s)

PCR: suitable

kategoria ekologicznej alternatywy

temp. przechowywania

−20°C

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Ta pozycja
S5692D2376G1041
concentration

10,000 × in DMSO

concentration

-

concentration

-

concentration

-

technique(s)

PCR: suitable

technique(s)

protein staining: suitable

technique(s)

-

technique(s)

protein staining: suitable

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

usage

 mL sufficient for 100 mini-gels

usage

-

usage

-

usage

-

Opis ogólny

SYBR® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.

Zastosowanie

SYBR® Green I nucleic acid gel stain has been used for:
  • the quantification of dsDNA
  • to stain DNA in polymerase chain reaction (PCR)
  • for comet assay technique
  • to assess spermatozoon membrane integrity
  • for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)
  • as a fluorescent dye in flow cytometry
  • real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA

Cechy i korzyści

  • An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
  • It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
  • It is less mutagenic than ethidium bromide in Ames tests
  • It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
  • Useful for many applications with a limited amount of DNA
  • The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
  • Removal of this stain in-gel digestion and ligation techniques is not needed
  • SYBR Green I is a greener alternative product to ethidium bromide for staining

Przechowywanie i stabilność

Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.

Informacje prawne

SYBR is a registered trademark of Life Technologies

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Cennik

Kod klasy składowania

10 - Combustible liquids

WGK

WGK 3

Temperatura zapłonu °F

201.2 °F - closed cup

Temperatura zapłonu °C

94 °C - closed cup

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificates of Analysis (COA)

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Przykład

T1503
Numer produktu
-
25G
Wielkość opakowania/ilość

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

wpisz jako 1.000309185)

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Klienci oglądali również te produkty

Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications
Zipper H, et al.
Nucleic Acids Research, 32(12), e103-e103 (2004)
P A Morin et al.
BioTechniques, 19(2), 223-228 (1995-08-01)
A method for resolving and visualizing genotypes for simple sequence repeat loci on short (20 cm) polyacrylamide gels is described. This method makes use of a modified vertical electrophoresis cell to allow rapid electrophoresis without variation in migration rates due
The in vitro effect of nonylphenol, propranolol, and diethylstilbestrol on quality parameters and oxidative stress in sterlet (Acipenser ruthenus) spermatozoa
Shaliutina O, et al.
Toxicology in vitro, 43, 9-15 (2017)
Loop mediated isothermal amplification of 5.8S rDNA for specific
detection of Tritrichomonas foetus
Jorge Oyhenart
Veterinary Parasitology, 193 (2013)
I Ortiz et al.
Animal reproduction science, 187, 74-78 (2017-10-19)
The aim of this study was to compare sperm DNA fragmentation of frozen-thawed epididymal sperm of dogs using the SCSA (Sperm Chromatin Structure Assay) and SCDt (Sperm Chromatin Dispersion test). For this purpose, epididymis from neutered dogs were minced and

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