N9162

Neocarzinostatin from Streptomyces carzinostaticus

≥90% (SDS-PAGE), ~0.5 mg/mL

• Synonym(s): Holoneocarzinostatin, NCS, NSC-69856, Zinostatin
• CAS Number: 9014-02-2
• Numer MDL: MFCD01778130
• NACRES: NA.77

Properties

• Poziom jakości: 200
• próba: ≥90% (SDS-PAGE)
• forma: solution
• stężenie: ~0.5 mg/mL
• spektrum działania antybiotyku: neoplastics
• Tryb działania: DNA synthesis | interferes
• transport: wet ice
• temp. przechowywania: 2-8°C
• InChI: 1S/C35H35NO12/c1-16-12-19(42-4)14-22-20(16)8-9-23(37)27(22)32(40)45-24-13-18-10-11-35(26-15-43-34(41)46-26)25(48-35)7-5-6-21(18)31(24)47-33-28(36-3)30(39)29(38)17(2)44-33/h8-9,12-14,17,21,24-26,28-31,33,36-39H,6,15H2,1-4H3/t17-,21?,24-,25-,26-,28-,29+,30-,31-,33-,35+/m1/s1
• InChI key: BLXZMHNVKCEIJX-PNKAXBHOSA-N

Description

Zastosowanie

Neocarzinostatin from Streptomyces carzinostaticus has been used:

• as a DNA damaging agent to treat human fibroblast GM0637 cells
• as a radiomimetic antibiotic to induce DNA damage
• in combination with Vγ2Vδ2 T cells to determine the cytotoxicity in ovarian cancer cells

Opakowanie

100 μg in glass bottle

Działania biochem./fizjol.

Neocarzinostatin is a protein-small molecule complex composed of an enediyne chromophore tightly bound to a 113 amino acid single chain protein. The complex possesses antiproliferative and antitumor activity. The chromophore is the active compound, which is responsible for DNA cleavage; while the apoprotein stabilizes and regulates the availability of the labile chromophore. NCS chromophore is bound non-covalently in a cleft of the binding protein and is dissociable. Upon addition of a thiol, the chromophore forms a highly reactive biradical species that can induce sequence-specific single and double strand breaks in DNA. Neocarzinostatin inhibits DNA synthesis and possesses antitumor activity in various human and animal tumors. NCS inhibits cellular proliferation by inducing G₂ cell cycle arrest and apoptosis in both human papillomavirus (HPV) positive and negative cell lines.

Neocarzinostatin is a radiomimetic antibiotic substance.

Postać fizyczna

Supplied as 100 μg (~9 nmoles) in a solution containing 20 mM MES buffer, pH 5.5.

Safety Information

• Kod klasy składowania: 12 - Non Combustible Liquids
• Temperatura zapłonu °F: Not applicable
• Temperatura zapłonu °C: Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)