Merck
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PHG0001

SAFC

HEPES

Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
Numer EC:
Numer MDL:
Identyfikator substancji w PubChem:
NACRES:
NA.21

pochodzenie biologiczne

synthetic

Poziom jakości

400

forma

powder

technique(s)

cell culture | mammalian: suitable

zanieczyszczenia

Endotoxin, microbial, and trace metals; tested

pH

4.7-5.3 (, )

useful pH range

6.8-8.2

pKa (25°C)

7.5

przydatność

suitable for manufacturing use

obecność zanieczyszczeń

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Opakowanie

100 g in poly bottle
5, 10, 25, 50 kg in poly drum

Zastosowanie

HEPES is a biological buffer often referred to as a “Good′s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high solution clarity.

HEPES is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

Kod klasy składowania

13 - Non Combustible Solids

WGK

WGK 1

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certificate of Analysis

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Certificate of Origin

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