SAB4300645

Sigma-Aldrich

Anti-GAPDH antibody produced in rabbit

affinity isolated antibody

Synonim(y):
Anti-MGC88685, Anti-GAPD, Anti-G3PD
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

100

forma przeciwciała

affinity isolated antibody

antibody product type

primary antibodies

klon

polyclonal

formularz

buffered aqueous solution

masa cząsteczkowa

~37 kDa

species reactivity

mouse, rat, human

stężenie

1 mg/mL

zastosowania

western blot: 1:500-1:1000

izotyp

IgG

sekwencja immunogenna

(P-A-K-Y-D)

białko sprzężone

unconjugated

numer dostępu NCBI

numer dostępu UniProt

wysyłka w ciągu

wet ice

temp. przechowywania

−20°C

Gene Information

human ... GAPDH(2597)

Opis ogólny

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein , that is located on chromosome 12p13. It is basically called as a glycolytic enzyme.

The antibody detects endogenous level of total GAPDH protein.

Immunogen

Peptide sequence around aa. 252-256 (P-A-K-Y-D), according to the protein NP_002037.2

Zastosowanie

Anti-GAPDH antibody has been used in western blotting.{60,61}

Działania biochem./fizjol.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) plays an important role in DNA repair. It is essential for the sixth step of glycolysis. It participates in transcriptional and posttranscriptional gene regulation. It also controls intracellular membrane trafficking and cell death. Accumulation of GAPDH results in mitochondrial dysfunction.

Cechy i korzyści

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Opis wartości docelowych

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Postać fizyczna

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certyfikat analizy

Świadectwo pochodzenia

Pingting Liu et al.
Disease models & mechanisms, 10(1), 53-62 (2016-12-10)
The S113R mutation (c.339T>G) (MIM #603690.0001) in SLC33A1 (MIM #603690), an ER membrane acetyl-CoA transporter, has been previously identified in individuals with hereditary spastic paraplegia type 42 (SPG42; MIM #612539). SLC33A1 has also been shown to inhibit the bone morphogenetic...
Yanwu Hu et al.
Molecular medicine reports, 13(3), 2899-2903 (2016-01-29)
Icariin, a flavonoid isolated from the traditional Chinese herbal medicine Epimedium brevicornum Maxim, has been shown to possess anti-inflammatory, anti‑oxidant and anti-atherosclerotic activities in vivo and in vitro. The aim of the present study was to investigate the effects of...
Role of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) in DNA Repair
Kosova AA, et al.
Biochemistry. Biokhimiia (2017)
Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Aggregation Causes Mitochondrial Dysfunction during Oxidative Stress-induced Cell Death
Nakajima H, et al.
The Journal of Biological Chemistry (2017)
Increased copy number of the TERT and TERC telomerase subunit genes in cancer cells
Cao Y, et al.
Cancer Science (2008)

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej