[Przejdź do zawartości](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/PL/pl) Produkty Koszyk0 PLPL Produkty ProduktyZastosowaniaUsługiZasobyWsparcie [Zaloguj się / Zarejestruj się](https://www.sigmaaldrich.com/oidc-sign-in) [Wyszukiwanie zamówienia](https://www.sigmaaldrich.com/PL/pl/order-lookup) [Szybkie zamówienie](https://www.sigmaaldrich.com/PL/pl/quick-order) Koszyk0 [Home](https://www.sigmaaldrich.com/PL/en)[Next-Generation Sequencing](https://www.sigmaaldrich.com/PL/en/applications/genomics/next-gen-sequencing)Custom Next-Gen Sequencing Oligos # Custom Next-Gen Sequencing Oligos __Section Overview__ - [Next-Gen Sequencing Oligos Specifications](https://www.sigmaaldrich.com#next-gen-sequencing-oligos-specifications) - [Functional Quality Control](https://www.sigmaaldrich.com#functional-quality-control) - [Other Ngs Oligonucleotides](https://www.sigmaaldrich.com#other-ngs-oligonucleotides) - [Product Listings](https://www.sigmaaldrich.com#product-listings) Regardless of next-generation sequencing (NGS) platform, universal and index adapter sequences are required for the proper assembly of sample fragments. Adapters – especially index adapters since they contain the MID (multiplex identifier) or barcode – containing too high a proportion of truncated sequences (unacceptably low purity) or too high a proportion of other adapter sequences (excessive cross contamination) can lead to compromised sequence read integrity (excessive adapter dimerization too) and improper sequence assembly, respectively, during multiplexing experiments. These types of problems are undetectable until the data analysis stage, which make them costly in terms of time and money. Therefore, the production process used for the adapter sequences is critical for a successful sequencing run. Our custom Next-Gen Sequencing Oligos (NGSO) are manufactured under rigorous conditions to ensure suitable purity and low cross contamination to meet research, commercial, and molecular diagnostic needs by eliminating adapter-quality-at-fault failed sequencing runs. Use the table below to decide which of the three options is appropriate to meet your needs. The primary selection criteria should be which cross-contamination ceiling is acceptable along with the quality management system (ISO 9001 for research and commercial needs; ISO 13485 for molecular diagnostic needs). When in doubt, select NGSO-Gold for Illumina instruments and NGSO-Silver for the Thermo Fisher (Ion Torrent™) instrument. [Order Now](https://www.sigmaaldrich.com/configurators/ngso) Next-Gen Sequencing Oligos Specifications __Option____NGSO-Bronze____NGSO-Silver____NGSO-Gold__ __Cross__ __Contamination1__ - <2% - <1% - <0.25% __Scale & Minimum__ __Yield2__ - 0.05 µmol (1 - 3 OD) - 0.2 µmol (3 - 8 OD) - 1.0 µmol (9 - 13 OD) - Inquire for larger scales - 0.2 µmol (10 nmol or 2 - 6   OD depending on sequence   length) - Inquire for larger scales - 0.2 µmol (3 - 8 OD) - 1.0 µmol (9 - 13 OD) - Or, final yields: 25, 50, 100,   250 & 500 nmol - Or, inquire for mg quantities __Purity__ - 70-85% - 65-80% - 85-95% __Sequence__ __Length__ - 10 - 80 bases - 10 - 60 bases - 10 - 80 bases __Modifications__ - 5’-Phosphate - Phosphorothioate - 5-Me-dC - 5’-Phosphate - Phosphorothioate - 5-Me-dC - 5’-Phosphate - Phosphorothioate - 5-Me-dC __Format__ - Supplied dry in tubes - Supplied in solution in   tubes or 96-well plates - Standard: 100 µM in water,   TE (10 mM Tris, pH 7.5-8.0,   1 mM EDTA ), or Tris   (10 mM Tris-HCl, pH 8.0) - Inquire: 30-150 µM in water   or 30-130 µM in   TE / Tris buffer - Mixing Available: Yes - Duplexing Available: No - Supplied dry or in water   / buffer in tubes   (pooling & aliquoting also   available) Note: In Solution option only available for US __Platform__ __Compatibility__ - Illumina, Thermo   Fisher (Ion Torrent™),   & PacBio™ - Thermo Fisher   (Ion Torrent™) - Illumina, Thermo Fisher   (Ion Torrent™), & PacBio™ __Quality Control__ - 100% Mass Spectrometry - Analytical HPLC   available - Certificate of Analysis   available - 100% Mass Spectrometry - Analytical HPLC available - Certificate of Analysis   available - 100% Mass Spectrometry - Analytical HPLC available - Certificate of Analysis   available __Quality__ __Management System__ - ISO 9001 - ISO 9001 - ISO 13485 __Typical Applications__ - Targeted sequencing   of select genes or   panels - SNP genotyping & variant   detection - Manufacturing of   commercial kits 1Values determined via independent sources that used adapters manufactured with these processes. Multiplex sequencing was performed using Illumina instruments. 2Expected yields are valid for oligonucleotides 20 bases or longer. ## Functional Quality Control[](https://www.sigmaaldrich.com) NGSO-Bronze, Silver, and Gold have undergone process validations to ensure that each provides adapters with cross contamination levels below the ceilings in the above table. However, prior to shipment, NGSO adapters as a matter of routine do not undergo functional testing with NGS to provide the actual cross contamination values. If you would like functional quality control of your adapters via NGS, please [send](mailto:dnaoligos@milliporesigma.com) requirements. We will perform a feasibility assessment (this includes consulting with you regarding the protocol), and if possible, provide a quotation with pricing and an estimated completion date. Testing will be performed on Illumina instruments. ## Other NGS Oligonucleotides[](https://www.sigmaaldrich.com) In addition to the adapters, the major sequencing platforms often require other types of oligonucleotides for additional steps in the workflow, e.g. amplification primers (__Figure 1__). These other oligonucleotides do not have to meet the same low cross-contamination ceiling as the adapters; therefore, they can be ordered as standard Custom DNA Oligos.  __Figure 1.__A generic next-generation sequencing workflow and the required oligonucleotides. However, we do recommend certain purifications based on the function of the oligonucleotides as shown in the table. | | | | | |--------------------------------------------------------------------|-------------------|----------------------------------|-------------------| | Recommended Purification for Non-Adapter Next-Gen Oligonucleotides | | | | | __Platform__ | __Illumina__ | __Thermo Fisher (Ion Torrent™)__ | __PacBio™__ | | __Amplification Primers__ | Cartridge or HPLC | Not available | Not applicable | | __Target Capture Probes__ | HPLC | HPLC | HPLC | | __Blocking Oligos__ | HPLC | HPLC | HPLC | | __Sequencing Primers__ | Cartridge or HPLC | Not applicable | Cartridge or HPLC | | __Multiplexing Primers__ | Not applicable | Not applicable | Cartridge or HPLC | ## Related Products Przepraszamy, wystąpił nieoczekiwany błąd Response not successful: Received status code 500 Click [here](https://www.sigmaaldrich.com/PL/en/products/molecular-biology-and-functional-genomics/oligos-and-qpcr-probes/custom-predesigned-dna-oligos-and-qpcr-probes) to order these additional, custom next-generation sequencing oligonucleotides. __Related Articles__ - [Benzonase® Nuclease for Microbiome Workflows](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/dna-and-rna-purification/benzonase-nuclease-microbiome-workflows) - [MetaPolyzyme, DNA Free Cell Lysis Enzymes for Microbiome Workflows](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/dna-and-rna-purification/metapolyzyme-dna-free-cell-lysis-enzymes-for-microbiome-workflows) - [Ultrapure vs. DEPC Treated Water for Molecular Biology Applications](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/dna-and-rna-purification/nuclease-free-water) - [ChIC and CUT&RUN Data Analysis Tool Frequently Asked Questions (FAQs)](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/next-gen-sequencing/chic-data-analysis-tool-faq) - [View More](https://www.sigmaaldrich.com/PL/en/search/facet-search?focus=sitecontent&term=facet-search) Góra __Zaloguj się, aby kontynuować__ Zaloguj się lub utwórz konto, aby kontynuować. 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