Merck

Glucose-6-phosphate tips the balance in modulating apoptosis in cerebellar granule cells.

FEBS letters (2015-02-04)
A Bobba, G Amadoro, G La Piana, V A Petragallo, P Calissano, A Atlante
ABSTRAKT

A metabolic shift from oxidative phosphorylation to glycolysis (i.e. the Warburg effect) occurs in Alzheimer's disease accompanied by an increase of both activity and level of HK-I. The findings reported here demonstrate that in the early phase of apoptosis VDAC1 activity, but not its protein level, progressively decreases, in concomitance with the physical interaction of HK-I with VDAC1. In the late phase of apoptosis, glucose-6-phosphate accumulation in the cell causes the dissociation of the two proteins, the re-opening of the channel and the recovery of VDAC1 function, resulting in a reawakening of the mitochondrial function, thus inevitably leading to cell death.

MATERIAŁY
Numer produktu
Marka
Opis produktu

Sigma-Aldrich
Anti-VDAC1 Antibody, clone N152B/23, clone N152B/23, from mouse
Sigma-Aldrich
Monoclonal Anti-Actin antibody produced in mouse, clone AC-40, ascites fluid