[Przejdź do zawartości](https://www.sigmaaldrich.com#main-content) [![Merck](https://www.sigmaaldrich.com/static/logos/purple/merck.svg)](https://www.sigmaaldrich.com/PL/pl) Produkty Koszyk0 PLPL Produkty ProduktyZastosowaniaUsługiZasobyWsparcie [Zaloguj się / Zarejestruj się](https://www.sigmaaldrich.com/oidc-sign-in) [Wyszukiwanie zamówienia](https://www.sigmaaldrich.com/PL/pl/order-lookup) [Szybkie zamówienie](https://www.sigmaaldrich.com/PL/pl/quick-order) Koszyk0 [Home](https://www.sigmaaldrich.com/PL/en)[Cloning & Expression](https://www.sigmaaldrich.com/PL/en/applications/genomics/cloning-and-expression)Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure) # Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure) ## DNA Polymerases (ultra pure) DNA Polymerases synthesize DNA from nucleotides. DNA polymerase is required for DNA replication, but also essential for many other activities in the cell, including genetic recombination, DNA repair, and reverse transcription. In molecular biology labs, DNA polymerases are used for applications such as cloning, PCR, and DNA sequencing. Each DNA polymerase has special characteristics, making it useful for specific applications. Knowing these characteristics enables you to pick the right ultra pure DNA Polymerase for your application. ### DNA Polymerase Selection Chart Product NumberProduct DescriptionNotable CharacteristicsApplication Suitability [KEM0026](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0026)Klenow (3’ → 5’ exo) - Deficient in both proofreading and nick translation nuclease activities - Moderate strand displacement activity - A-tailing for NGS - Strand displacement amplification - DNA labeling [KEM0027](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0027)Klenow (3’ → 5’ exo) [KEM0028](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0028)phi29 DNA Polymerase (High concentration) - Highly processive - Proofreading activity (3’ → 5’) - Powerful strand displacement activity - Whole Genome Amplification [KEM0029](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0029)phi29 DNA Polymerase (Low concentration) [KEM0030](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0030)DNA Polymerase I - Exhibits 3’ → 5’ synthesis activity - Exonuclease activities (both 3’ → 5’ and 5’ → 3’) - Second strand cDNA synthesis - Nick-translation - DNA labeling [KEM0031](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0031)Klenow Fragment - Exhibits synthesis and proofreading (3’ → 5’) nuclease activities - Moderate strand displacement activity - DNA blunting by fill-in of 5’ overhang - Second strand cDNA synthesis - Sequencing - Site-directed mutagenesis [KEM0032](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0032)Terminal deoxynucleotidyl Transferase (TdT) - Template-independent - Catalyzes addition of deoxynucleotides to 3’OH of ss- or ds-DNA - Homopolymeric tailing to the 3’OH - TUNEL assay - 5’ RACE - Labeling of 3’ end [KEM0033](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0033)T4 DNA Polymerase - Catalyzes extension of a primed DNA template (5’ → 3’ direction) - Powerful 3’ → 5’ exonuclease activity - Lacks inherent 5’ → 3’ exonuclease or strand displacement activity - DNA end-repair by fill-in of 5’ overhang - Removal of 3’ overhang [KEM0034](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0034)Mako DNA Polymerase (3’ → 5’ exo) - Catalyzes extension of a primed DNA template (5’ → 3’ direction) - Protocols requiring no 3’ → 5’ or 5’ → 3’ exonuclease activity - Applications requiring no strand displacement activity [KEM0035](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0035)Manta 1.0 DNA Polymerase (High concentration) - Thermophilic enzyme - Deficient in both proofreading and nick translation nuclease activities - Applications requiring an enzyme with thermo-stable, strong strand replacement activity - Applications requiring a Bst I large fragment equivalent - SDA assy - LAMP assay - NEAR assay [KEM0036](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0036)Manta 1.0 DNA Polymerase (Low concentration) ## DNA Ligases (ultra pure) DNA Ligases form phosphodiester bonds between DNA strands, joining them together. DNA Ligase is responsible for repairing breaks in single and double stranded DNA. In molecular biology labs, DNA Ligase is commonly used for molecular cloning applications. Each DNA Ligase has special characteristics, making it useful for specific applications. Knowing these characteristics enables you to pick the right ultra pure DNA Ligase for your application. ### DNA Ligase Selection Chart Product DescriptionT4 DNA Ligase T4 DNA Ligase (Rapid) T3 DNA Ligase T7 DNA Ligase *E. coli* DNA Ligase Products No.[KEM0020](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0020)[KEM0019](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0019)[KEM0017](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0017)[KEM0018](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0018)[KEM0025](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0025) Units 150,000240,000900,000900,0002,500 Concentration120,000 U/mL600,000 U/mL3,000,000 U/mL3,000,000 U/mL40,000 U/mL Cofactor RequiredATPATPATPATPNAD Recommended for Cloning?✓✓ Recommended for cohesive ends✓✓✓✓✓ Recommended for blunt ends✓✓✓✓✓ Recommended for nicks in dsDNA✓✓✓✓✓ Recommended for joining of RNA & DNA hybrids✓✓ Application Suitability - Restriction cloning - TA cloning - Adapter ligation - NGS library construction - Restriction cloning - TA cloning - Adapter ligation - Applications requiring good activity under high ionic strength (1M NaCl) - TALE - Applications requiring high cohesive end ligation efficiency - cDNA cloning by replacement synthesis ## Nucleic Acid Modifying Enzymes (ultra pure) Many different enzymes exist that are able to modify DNA, RNA, and RNA:DNA hybrids. In molecular biology labs, these modifying enzymes are commonly used for molecular cloning and sequencing applications. Each enzyme has special characteristics, making it useful for specific applications. Knowing these characteristics enables you to pick the right ultra pure Nucleic Acid Modifying Enzyme for your application. ### Nucleic Acid Modifying Enzyme Selection Chart Product No.Product DescriptionNotable CharacteristicsApplication Suitability [KEM0006](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0006)T4 Polynucleotide Kinase - Catalyzes the transfer and exchange of ATP to 5’OH of ss- or ds-DNA and RNA - End-labeling DNA or RNA for probes - End-labeling DNA or RNA for sequencing - Addition of 5’ phosphates to oligonucleotides for subsequent ligation - Removal of 3’ phosphoryl groups [KEM0001](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0001)Uracil DNA Glycosylase (UDG) - Catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar (leaving an abasic site) - Removal of uracil from DNA - Control of carry-over contamination in PCR [KEM0002](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0002)Thermolabile Uracil DNA Glycosylase (UDG) - Catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar (leaving an abasic site) - Completely inactivated in 1x reaction buffer at 50°C for 10 minutes. [KEM0004](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0004)Exonuclease III - Digests one strand of the dsDNA at a time - Digests the RNA strand of RNA:DNA hybrids - Removal of nucleotide(s) from duplex DNA in the 3’ → 5’ direction [KEM0005](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0005)Lambda Exonuclease - Highly processive 5’ → 3’ double-stranded exonuclease - Degrades one strand of the duplex - Highly processive 5’ → 3’ exonuclease - Applications requiring activity on blunt and 5’ recessed ends [KEM0009](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0009)End-Repair Mix (High Concentration) - Mix of T4 DNA Polymerase and T4 Polynucleotide Kinase - Converts to blunt-ended DNA - Converting DNA containing damaged or incompatible 5’ and/or 3’ protruding ends to 5’ phosphoryleted, blunt-ended DNA [KEM0010](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0010)End-Repair Mix (Low Concentration) [KEM0011](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0011)10x Uracil Cleavage System - Generates a single nucleotide gap at uracil residues - Excising uracil residues from DNA [KEM0014](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0014)Poly(A) Polymerase - Catalyzes the addition of AMP (from ATP) to the RNA 3’OH - Labeling of RNA with ATP or cordycepin - Poly(A) tailing of RNA for cloning or affinity purification - Translation of RNA transferred into eukaryotic cells [KEM0015](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0015)RNase H - Degrades the RNA strand of RNA:DNA hybrids - Removal of mRNA during second-strand cDNA synthesis - Cleavage of RNA from RNA:DNA hybrids ## RNA Ligases (ultra pure) RNA Ligases form phosphodiester bonds between RNA strands, joining them together. In molecular biology labs, RNA Ligase is commonly used for mutagenesis of RNA, and end-labeling applications. Each RNA Ligase has special characteristics, making it useful for specific applications. Knowing these characteristics enables you to pick the right ultra pure RNA Ligase for your application. | | | | | |---------------------------------------------|---------------------------------------------------------------------|---------------------------------------------------------------------|---------------------------------------------------------------------| | Product Description | T4 RNA Ligase I | T4 RNA Ligase 2 | T4 RNA Ligase 2 (truncated) | | Product No. | [KEM0021](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0021) | [KEM0024](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0024) | [KEM0023](https://www.sigmaaldrich.com/PL/en/product/sigma/KEM0023) | | Units | 10,000 | 4,500 | 500 | | Concentration | 20,000 U/mL | 30,000 U/mL | 5,000 U/mL | | Cofactor Required | ATP | ATP | None | | Recommended for nicks in dsDNA? | | ✓ | | | Recommended for joining of RNA:DNA hybrids? | | ✓ | | | Recommended for labeling of RNA 3’ termini? | ✓ | | ✓ | | Recommended for joining ssDNA? | ✓ | | | | Recommended for joining ssRNA? | ✓ | | | __Related Articles__ - [Introduction to Cell Transfection](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/transfection-reagents) - [Reverse Transfection of Plasmid DNA](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/xtg-reverse-transfect-pdna) - [3xFLAG® System Expression Vectors for Ultra-Sens](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/cloning-and-expression/3x-flag) - [Blue-White Screening & Protocols for Colony Selection](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/cloning-and-expression/blue-white-screening) - [Bacterial Transformation Protocols](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/cloning-and-expression/competent-cells) - [Genotypes, Phenotypes and Markers](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/cloning-and-expression/definitions-and-genotypes-of-e-coli) - [Restriction Enzyme Cloning Glossary](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/cloning-and-expression/estriction-enzyme-cloning-manual-glossary) - [How Transfection Works](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/cloning-and-expression/how-transfection-works) - [View More](https://www.sigmaaldrich.com/PL/en/search/facet-search?focus=sitecontent&term=facet-search) Góra __Zaloguj się, aby kontynuować__ Zaloguj się lub utwórz konto, aby kontynuować. 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