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With personal consultation from our expert, technical support scientists, your request, including all sequences, will be MIQE compliant and returned to you within 24-48 hours. ### Specifications Design Modules__PCR, qPCR & dPCR Applications__ - SYBR®Green I Primers - Dual-Labeled Probes1 - Molecular Beacons2 - LightCycler® Probes3 - Traditional PCR - SNP detection - Allele discrimination - Pathogen detection - Multiplexing - Viral load quantification - Gene expression analysis - Gene copy determination - Endpoint genotyping - Methylation analysis - High-resolution melting analysis - Transcript detection across exon junctions - Haplotyping - Northern blotting - Southern blotting __Other Applications__ - FISH (fluorescence in situ hybridization) - ASO (antisense oligonucleotides)   __Modifications__ - Add Locked Nucleic Acid to any of the above design modules - Add MGB:EDQ (Minor Groove Binder:Eclipse Dark Quencher) to Dual-Labeled Probes 1 Also known as hydrolysis probes, which are used in the 5’ nuclease assay 2 Also known as hybridization probes 3 Also known as dual-hybridization probes Our consultative service uses Beacon Designer™ from Premier Biosoft International. Blastn and mfold are used to determine assay specificity. ## Materials Przepraszamy, wystąpił nieoczekiwany błąd Response not successful: Received status code 500 __Related Articles__ - [Milli-Q® IQ 7000 Ultrapure Water System](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/milli-q-iq-7000) - [Milli-Q® IQ 7003/05/10/15 Ultrapure & Pure Lab Water Purification System](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/analytical-chemistry/wet-chemical-analysis/milli-q-iq7003-7005) - [Custom Oligos for Commercial Use](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/iso-oligos) - [Custom LAMP Primers in Partnership with New England Biolabs](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/pcr/custom-lamp-primers) - [Easy Oligo](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/pcr/easy-oligo) - [Fast siRNA](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/pcr/fast-sirna-oligos) - [Scalable, High-Quality Custom Oligonucleotides for Commercial PCR, qPCR & NGS](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/pcr/iso-oligos) - [KOD One™ PCR Master Mix for Ultra-Fast, High-Fidelity PCR](https://www.sigmaaldrich.com/PL/en/technical-documents/technical-article/genomics/pcr/kod-one-pcr-master-mix) - [View More](https://www.sigmaaldrich.com/PL/en/search/facet-search?focus=sitecontent&term=facet-search) __Related Product Categories__ - [NGS Library Preparation](https://www.sigmaaldrich.com/PL/en/products/molecular-biology-and-functional-genomics/ngs-library-prep) Góra __Zaloguj się, aby kontynuować__ Zaloguj się lub utwórz konto, aby kontynuować. 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