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[Home](https://www.sigmaaldrich.com/US/en)[Gene Expression & Silencing](https://www.sigmaaldrich.com/US/en/applications/genomics/gene-expression-and-silencing)MISSION® Application Data
# MISSION® Application Data
We are committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries. Below are links to data pieces and articles on various topics of interest. If you would like us to post your data (specific applications, validation of gene targets, etc.) on our site, please send us your experimental summary and data by e-mail.
#### [](https://www.sigmaaldrich.com)Posters – MISSION® R&D
- A Screen of MISSION® shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in A549 Paclitaxel Sensitivity
- Gene Silencing in Primary Astrocyte Cells using MISSION® TRC shRNA Lentiviral Particles (338 Kb PDF)
- [Reproducible, Long-term Silencing in a High-throughput Lentiviral shRNA Screen](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/documents/long-term-silencing.pdf) (211 Kb PDF)
- [Verification of RNA Interference at the Protein Level via Quantitative Proteomics Analysis](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/229/578/rnai_ascb_2005.pdf) (374 Kb PDF)
- [Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/262/142/shrna_library_efficacy.pdf) (357 Kb PDF)
- Efficient Gene Silencing with High Selectivity using MISSION® TRC shRNA Lentiviral Vectors
#### [](https://www.sigmaaldrich.com)Posters – External Researchers
- [Lentiviral shRNA Screen of Multidrug Resistant Associated Genes Identifies PRP-4 as a New Regulator of Chemoresistance in Human Ovarian Cancer](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/documents/lentiviral-shrna-screen-2008-aacr-poster.pdf) (635 Kb PDF)
Zhenfeng Duan, M.D. Ph.D. – Harvard Medical School
- [shRNA Phenotypic Screen To Identify Gene Targets Contributing to Androgen Independent Prostate Cancer Cell Growth](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/documents/shrna-phenotypic-screen.pdf) (989 Kb PDF)
Daniel Gioeli Ph.D. – University of Virginia
- [Mesenchymal Stem Cells: Cell Death or Survival? Interaction with Other Stress Pathways](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/371/603/poster-lisa-oliver.pdf) (887 Kb PDF)
by Oliver L. and Vallette FM, Center for Cancer Research Nantes - Angers INSERM UMR 892, University of Nantes, France
### Selected Data Figures
Data typeFormatGene TargetsCell LinesDetection LevelProvided by
Lentivirus is stable after 2.5 yearsViral ParticlesN/AA549Phenotypic*Our internal operations*
Long Term, up to 1 yr, Gene Silencing with shRNAViral ParticlesARGHDIAA549mRNA*Our internal R&D*
Gene Target ValidationViral Particles, TransductionCDH1Human colorectal cancer cell line (coll5)Protein*Elena Sancho, PhD and Eduard Batlle, PhD*
*Barcelona Institute for Research in Biomedicine*
Stable Cell Line/ Signaling PathwayViral Particles, TransductionZFP36 (TTP)A549mRNA & Protein*Kathleen Smoak, PhD and John A Cidlowski, PhD*
*Laboratory of Signal Transduction, National Institute of Environmental Health Services, National Institutes of Health*
Efficacy, Specificity and EfficiencyViral Particles, Transductionp38α, p38γ, JNK2, MAP3K4, JAK1, AKT1, AKT2, AKT3Primary human astrocytes, A549, HepG2, HCT116, Panc1, HeLamRNA*Our internal R&D*
Primary Astrocyte Cells (Neuronal Cells)Viral Particles, Transductionp38α, p38γ, JNK2, MAP3K4Primary human astrocytesmRNA*Our internal R&D*
Specificity in Targeting IsoformsViral Particles, TransductionAKT1, AKT2, AKT3A549mRNA*Our internal R&D*
Gene Target ValidationViral Particles, Transductionc-MYCHuman lung cancer cell line (H1299)mRNA & Protein*Steven B. McMahon, Ph.D.*
*The Wistar Institute*
Demonstration of 3' UTR Utility, Knockdown of Overexpressed ProteinPlasmid, TransfectionFBXO6COS-7Protein*Kevin Glenn, M.D.*
*Carver College of Medicine, University of Iowa*
Efficiency of MISSION® TurboGFP™ Transduction ControlViral Particles, TransductionN/APrimary BJ FibroblastsProtein (FACS)*Sheila Stewart, Ph.D.*
*Washington University*
Interferon ResponseViral Particles, TransductionCSK, AUR/KB, TYK2, YES1, AKT3HT29mRNA*Cell, 124, pp. 1283-1298. 20061*
Stability of pLKO.1-puroPlasmid DNAN/AN/ARestriction Digest*Cell, 124, pp. 1283-1298. 20061*
Dose-ResponseViral Particles, TransductionHCK, PDGFRB, TIE1, BCR, YES1, MAP2K4, PTK7, KIS, PRKACB, MAKHT29Cell Viability, Mitotic Index*Cell, 124, pp. 1283-1298. 20061*
Efficacy in Cell TypesViral Particles, TransductionN/ASee TableN/A*Compilation*
High-Throughput Gene Target ValidationViral Particles, Transduction60 Different TargetsA549mRNA*Our internal R&D*
High-Throughput ScreeningViral Particles, TransductionHuman Tumor Suppressor Gene Family SetA549Cell Viability Assay*Our internal R&D*
High-Throughput Lentiviral Particle ProductionViral Particles6000+ individual clonesN/Ap24 Assay*Our internal operations*
### Publications
1\.
Duan Z, Weinstein EJ, Ji D, Ames RY, Choy E, Mankin H, Hornicek FJ. 2008. Lentiviral short hairpin RNA screen of genes associated with multidrug resistance identifies PRP-4 as a new regulator of chemoresistance in human ovarian cancer. Mol Cancer Ther. 7(8):2377-2385. [https://doi.org/10.1158/1535-7163.mct-08-0316](https://doi.org/10.1158/1535-7163.mct-08-0316)
2\.
Bauer JA, Ye F, Marshall CB, Lehmann BD, Pendleton CS, Shyr Y, Arteaga CL, Pietenpol JA. 2010. RNA interference (RNAi) screening approach identifies agents that enhance paclitaxel activity in breast cancer cells. Breast Cancer Res. 12(3): [https://doi.org/10.1186/bcr2595](https://doi.org/10.1186/bcr2595)
3\.
Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, Piqani B, Eisenhaure TM, Luo B, Grenier JK, et al. 2006. A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen. Cell. 124(6):1283-1298. [https://doi.org/10.1016/j.cell.2006.01.040](https://doi.org/10.1016/j.cell.2006.01.040)
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- [Introduction to Cell Transfection](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/transfection-reagents)
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- [Safe Lentivirus Handling](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/safe-handling-of-lentivirus)
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