cell culture | mammalian: suitable
glucose: no (Dextro)
sodium pyruvate: no
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12 - Non Combustible Liquids
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
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The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.
This is a 1X concentration. The 10X concentration is listed under product D1408.
The pH will be in the range of 7.1-7.5, according to the specification sheet.
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The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted. They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour. The cells are counted and %viability determined. The cells are then cultured for an additional 7 hours (total of 8 hours). The cells are counted again and the % viability determined. The viability at 1 hr has to be >95% of the original viability. The viability at 8 hrs must be >75% of the original viability.
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