WGA2

GenomePlex^® Complete Whole Genome Amplification (WGA) Kit

Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue

• Synonym(s): Whole genome amplification kit
• NACRES: NA.55

Properties

• Quality Level: 200
• technique(s): whole genome amplification: suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

GenomePlex^® Complete Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. It contains everything required for whole genome amplification including an optimized enzyme, WGA DNA Polymerase. It can be used in several applications and is suitable for use with purified genomic DNA from a variety of sources including blood cards, whole blood, buccal swabs, soil, plant, and serum. It allows the researcher to generate a representative, ~500-fold amplification of genomic DNA.

Application

GenomePlex^® Complete Whole Genome Amplification (WGA) Kit has been used in the amplification of DNA. This kit is also suitable for use with downstream applications including:

• microarray analysis
• SNP analysis
• STR analysis
• DNA archiving

Features and Benefits

• Higher yield from minimal template: Amplification of nanogram amounts of genomic DNA to microgram yields (around 10 μg) in less than about three hoursrs
• Nanograms of samples can be preserved at –20 °C for future use
• Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, & formalin-fixed paraffin-embedded tissue (FFPE)
• Whole-genome amplification (WGA) DNA polymerase increases the amplification accuracy
• Whole-genome representation with no detectable allele bias
• Compatible with many downstream applications such as TaqMan^® assays, single nucleotide polymorphism (SNP) analysis, comparative genomic hybridization (CGH) analysis

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

GenomePlex is a registered trademark of Takara Bio USA, Inc.

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Kit Components Also Available Separately

• W4502 Water, Nuclease-Free Water, for Molecular Biology

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3