Merck
All Photos(3)

227935

Sigma-Aldrich

Azure B

prepared by direct synthesis

Synonym(s):
Azure I, Methyleneazure, N,N,N′-Trimethylthionin
Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
Molecular Weight:
305.83
Colour Index Number:
52010
Beilstein:
3922630
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

assay

>85.0% (HPLC)

form

powder

mp

205-210 °C (dec.) (lit.)

solubility

water: 1 mg/mL, blue to very deep blue

λmax

648 nm

ε (extinction coefficient)

≥13000 at 241-247 nm
≥36000 at 287-293 nm
≥67000 at 638-644 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]

InChI

1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H

InChI key

DNDJEIWCTMMZBX-UHFFFAOYSA-N

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Related Categories

Application

Azure B has been used in M′Fadyean stain to visualize Bacillus anthracis capsule.

Packaging

5, 100 g in glass bottle

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localization of nucleic acids and lignin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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