Merck
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608041

Sigma-Aldrich

L-Lysine-13C6,15N2 hydrochloride

99 atom % 15N, 99 atom % 13C, 95% (CP)

Synonym(s):
13C and 15N Labeled Lys, SILAC Amino Acid, (S)-2,6-Diaminohexanoic acid-13C6,15N2 hydrochloride, 13C and 15N Labeled lysine hydrochloride
Linear Formula:
H215N(13CH2)413CH(15NH2)13CO2H · HCl
CAS Number:
Molecular Weight:
190.59
MDL number:
PubChem Substance ID:
NACRES:
NA.12

Quality Level

isotopic purity

99 atom % 13C
99 atom % 15N

assay

95% (CP)

form

solid

optical activity

[α]25/D +20.7°, c = 2 in 5 M HCl

technique(s)

bio NMR: suitable
protein expression: suitable

mp

263-264 °C (dec.) (lit.)

mass shift

M+8

SMILES string

Cl.[15NH2][13CH2][13CH2][13CH2][13CH2][13C@H]([15NH2])[13C](O)=O

InChI

1S/C6H14N2O2.ClH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H/t5-;/m0./s1/i1+1,2+1,3+1,4+1,5+1,6+1,7+1,8+1;

InChI key

BVHLGVCQOALMSV-GSMNGUNQSA-N

Related Categories

General description

Lysine is a basic ketogenic amino acid with a protonated alkyl amino group. Lysine degradation results in the formation of acetoacetate. Acetylation/deacetylation of lysine residues in histones is a mechanism to regulate chromatin organization in eukaryotes. Isotope labeled amino acids are required for the production of isotopically labeled proteins.

Application

Pulsed Stable Isotope labeling of amino acid has been used to study host-cell function, survival, the precise intracellular pathways in protein synthesis of HIV-1 infected human monocytederived macrophages.
  • L-Lysine-13C6,15N2 hydrochloride has been used in pSILAC (pulsed stable isotope labeling by amino acids in cell culture) mass spectrometry to study protein profile in cells transfected with microRNAs.
  • It has been used in SILAC isotopic labeling for the characterization of lysine racemase.
  • It has been used in SILAC labeling for phosphoproteomic measurements in neuro-2a cells treated with and without GFKP-19 (2-pyrrolidone derivative).

Packaging

1, 2 g in glass bottle
100, 250 mg in glass bottle
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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