608041

L-Lysine-¹³C₆,¹⁵N₂ hydrochloride

99 atom % ¹⁵N, 99 atom % ¹³C, 95% (CP)

• Synonym(s): ¹³C and ¹⁵N Labeled Lys, SILAC Amino Acid, (S)-2,6-Diaminohexanoic acid-¹³C₆,¹⁵N₂ hydrochloride, ¹³C and ¹⁵N Labeled lysine hydrochloride
• Linear Formula: H₂¹⁵N(¹³CH₂)₄¹³CH(¹⁵NH₂)¹³CO₂H · HCl
• CAS Number: 1200447-00-2
• Molecular Weight: 190.59
• MDL number: MFCD00144647
• PubChem Substance ID: 24881987
• NACRES: NA.12

Properties

• Quality Level: 200
• isotopic purity: 99 atom % ¹³C; 99 atom % ¹⁵N
• assay: 95% (CP)
• form: solid
• optical activity: [α]25/D +20.7°, c = 2 in 5 M HCl
• technique(s): bio NMR: suitable; protein expression: suitable
• mp: 263-264 °C (dec.) (lit.)
• mass shift: M+8
• SMILES string: Cl.[15NH2][13CH2][13CH2][13CH2][13CH2][13C@H]([15NH2])[13C](O)=O
• InChI: 1S/C6H14N2O2.ClH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H/t5-;/m0./s1/i1+1,2+1,3+1,4+1,5+1,6+1,7+1,8+1;
• InChI key: BVHLGVCQOALMSV-GSMNGUNQSA-N

Description

General description

Lysine is a basic ketogenic amino acid with a protonated alkyl amino group. Lysine degradation results in the formation of acetoacetate. Acetylation/deacetylation of lysine residues in histones is a mechanism to regulate chromatin organization in eukaryotes. Isotope labeled amino acids are required for the production of isotopically labeled proteins.

Application

Pulsed Stable Isotope labeling of amino acid has been used to study host-cell function, survival, the precise intracellular pathways in protein synthesis of HIV-1 infected human monocytederived macrophages.

• L-Lysine-¹³C₆,¹⁵N₂ hydrochloride has been used in pSILAC (pulsed stable isotope labeling by amino acids in cell culture) mass spectrometry to study protein profile in cells transfected with microRNAs.
• It has been used in SILAC isotopic labeling for the characterization of lysine racemase.
• It has been used in SILAC labeling for phosphoproteomic measurements in neuro-2a cells treated with and without GFKP-19 (2-pyrrolidone derivative).

Packaging

1, 2 g in glass bottle

100, 250 mg in glass bottle

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable