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2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl, DPPH, 1,1-Diphenyl-2-picrylhydrazyl radical
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:






~135 °C (dec.) (lit.)

storage temp.


SMILES string




InChI key


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General description

2,2-diphenyl-1-picrylhydrazyl is a free radical, which shows hydrogen acceptor ability towards antioxidants. Hence, it is commonly used in DPPH assay for measuring the antioxidant activity of different natural samples such as wine, fruits, herbal tea etc.


1, 5 g in glass bottle

Other Notes

Free radical

Signal Word


Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Self-react. C - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

4.1A - Other explosive hazardous materials



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More documents

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the solubility of 2,2-Diphenyl-1-picrylhydrazyl, DPPH, Product D9132?

    We would expect this material to be soluble in chloroform and methanol. Quality control testing shows that a concentration of about 50mg/mL in chloroform can be achieved.

  6. At what wavelength is the absorbance maximum (lambda max) for 2,2-Diphenyl-1-picrylhydrazyl, DPPH, Product D9132?

    Using chloroform as the solvent, the lambda max is approximately 332 nm.

  7. What is the extinction coefficient of 2,2-Diphenyl-1-picrylhydrazyl, DPPH, Product D9132?

    The millimolar extinction coefficient (EmM) has been reported to be 19.07 in chloroform, measured at a wavelength of 332 nm. Reference: Organic Electronic Spectra Data (OESD), 1, p. 753 (1946-1952).

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Determination of in vitro antidiabetic effects, antioxidant activities and phenol contents of some herbal teas.
Buyukbalci A and Sedef Nehir El.
Plant Foods For Human Nutrition, 63(1), 27-33 (2008)
Influence of solvent composition on antioxidant potential of model polyphenols and red wines determined with 2, 2-diphenyl-1-picrylhydrazyl.
Bertalanic L, et al.
Journal of Agricultural and Food Chemistry, 60(50), 12282-12288 (2012)
Studies on the antioxidants. XIII. Hydrogen donating capability of antioxidants to 2, 2-diphenyl-1-picrylhydrazyl.
KuREcHI T, et al.
Chemical & Pharmaceutical Bulletin, 28(7), 2089-2093 (1980)
Višnja Stepanić et al.
Antioxidants (Basel, Switzerland), 8(4) (2019-04-10)
Chalcones are polyphenolic secondary metabolites of plants, many of which have antioxidant activity. Herein, a set of 26 synthetic chalcone derivatives with alkyl substituted pyrazine heterocycle A and four types of the monophenolic ring B, were evaluated for the potential
Thamizhiniyan Venkatesan et al.
Journal of pharmaceutical analysis, 9(3), 193-200 (2019-07-13)
Pinus densiflora needle extract (PDNE) is widely reported to have many pharmacological activities including antioxidant potential. However, the solvent system used for extraction greatly affects its antioxidant quality. Hence, in the present study, we investigated the effect of a different

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