All Photos(6)



Anti-Bak Antibody, NT

Upstate®, from rabbit

Apoptosis regulator BAK, BCL2-antagonist/killer 1, BCL2-like 7 protein, Bcl-2 homologous antagonist/killer, Bcl-2-like 7 protein, pro-apoptotic protein BAK

biological source


Quality Level

antibody form

purified antibody

antibody product type

primary antibodies



species reactivity

mouse, human, rat




immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable



NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... BAK1(578)

General description

Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.


Specific for Bak, MW ~28 kDa.


Synthetic peptide (SEEQVAQQDTEEVFRSC) corresponding to amino acid residues 23-38 of human Bak with a cysteine residue added to the C-terminus for conjugation to KLH.
Epitope: N-terminus


Research Sub Category
BCL2 & Inhibition
Detect Bak using this Anti-Bak Antibody, NT validated for use in IC, IH, IP & WB.
Research Category
Apoptosis & Cancer
Immunohistochemistry (Paraffin):
5 μg/mL of a previous lot detected Bak in paraffin-embedded rat kidney tissue.

4 μg of a previous lot immunoprecipitated Bak from 500 μg mouse 3T3 cell lysate.


Evaluated by Western Blot on HEK293 lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected Bak on 10 µg of HEK293 lysates.

Target description

28 kDa


Replaces: 04-433

Physical form

Format: Purified
Purified rabbit polyclonal in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen liquid.
Protein A purified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Included Positive Antigen Control: Catalog # 12-301, non-stimulated human A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Molecular regulation of DNA damage-induced apoptosis in neurons of cerebral cortex.
Martin, LJ; Liu, Z; Pipino, J; Chestnut, B; Landek, MA
Cerebral Cortex (1991)
Anna V Miller et al.
PloS one, 8(4), e60685-e60685 (2013-04-12)
Paclitaxel (Taxol)-induced cell death requires the intrinsic cell death pathway, but the specific participants and the precise mechanisms are poorly understood. Previous studies indicate that a BH3-only protein BIM (BCL-2 Interacting Mediator of cell death) plays a role in paclitaxel-induced
mRNA for N-Bak, a neuron-specific BH3-only splice isoform of Bak, escapes nonsense-mediated decay and is translationally repressed in the neurons.
Jakobson, M; Lintulahti, A; Arumae, U
Cell Death & Disease null
Bim is the key mediator of glucocorticoid-induced apoptosis and of its potentiation by rapamycin in human myeloma cells.
Lopez-Royuela N, Balsas P, Galan-Malo P, Anel A, Marzo I, Naval J
Biochimica et Biophysica Acta null
Stephanie Einsele-Scholz et al.
Journal of cell science, 129(11), 2213-2223 (2016-04-15)
The pro-apoptotic multidomain Bcl-2 proteins Bax and Bak (also known as BAK1) are considered the gatekeepers of the intrinsic pathway of apoptosis by triggering the mitochondrial release of cytochrome c The role of the third Bax- and Bak-homologous multidomain protein

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service