Merck
All Photos(2)

1.40197

Millipore

EcoCult® Half FRASER broth with antibiotics

ISO 11290, APHA, suitable for microbiology, pathogen testing

Synonym(s):
Demi FRASER broth with antibiotics

Agency

APHA
ISO 11290

Quality Level

manufacturer/tradename

EcoCult®

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

10-30°C

suitability

Listeria spp. (spp.)

General description

EcoCult® Half FRASER (Demi FRASER) broth has high nutrient content and a large buffer capacity that creates optimum growth conditions for Listeria spp. Lithium chloride, nalidixic acid, and acriflavine hydrochloride largely inhibit the growth of accompanying bacteria. The detection of the β-D-glucosidase activity is possible by the addition of aesculin and ammonium iron (III) citrate, forming a black complex of aesculin with iron (III) ions. A black coloration can develop during incubation, but this reaction is not exclusive to Listeria spp. and cultures that show no blackening cannot be assumed to be free of Listeria spp. According to EN ISO 11290-1, every primary and secondary enrichment in Fraser broth has to be sub-cultured on selective plating media. The EcoCult® Half FRASER (Demi FRASER) Broth with antibiotics already contains the antibiotics, so it is only necessary to add the Ammonium iron (III) citrate (1.00092) for the preparation of the primary selective enrichment Half Fraser broth. For the secondary enrichment step, Fraser broth can be prepared by the addition of one additional vial reconstituted FRASER Listeria selective supplements (1.00093) per 500 mL prepared Half Fraser Broth.

Application

EcoCult® Half FRASER (Demi FRASER) broth with antibiotics is used for the primary selective enrichment of Listeria spp. (including L. monocytogenes) from food and animal feed as well as from environmental samples in the area of food production and food handling and other materials.

Features and Benefits

  • Compliance to major regulatory standards ISO, FDA-BAM, EPA or USP
  • Clear labeling and Certificate of Analysis to check compliance at a glance
  • Reliable and accurate quality in powder format
  • Cost-effective pricing

Legal Information

ECOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificate of Analysis

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Certificate of Quality

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