Merck
All Photos(1)

164545

Sigma-Aldrich

7-Amino-4-methylcoumarin - CAS 26093-31-2 - Calbiochem

Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity.

Synonym(s):
AMC, Coumarin 120, 7-Amino-4-methylcoumarin - CAS 26093-31-2 - Calbiochem
Empirical Formula (Hill Notation):
C10H9NO2
CAS Number:
Molecular Weight:
175.18
MDL number:

Quality Level

assay

≥98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

yellow

solubility

DMSO: 10 mg/mL
acetone: 10 mg/mL
DMF: soluble

fluorescence

λex 365-380 nm
λem 430-460 nm

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3

InChI key

GLNDAGDHSLMOKX-UHFFFAOYSA-N

General description

Reagent used to prepare fluorogenic 7-amino-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzymes (excitation maximum: ~365-380 nm; emission maximum: ~430-460 nm). Also useful as a reference standard in enzyme assays.
Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity. Useful as a reference standard in enzyme assays.

Packaging

10 mg in Plastic ampoule
Packaged under inert gas

Biochem/physiol Actions

Primary Target
Detection of proteolytic enzyme activity
Product does not compete with ATP.
Reversible: no
Cell permeable: no

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). DMF and DMSO stock solutions are stable for up to 6 months at -20°C. Acetone stock solutions are stable for up to 1 week at 4°C or -20°C.

Other Notes

Kanaoka, Y., et al. 1982. Chem. Pharm. Bull. 30, 1485.
Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service