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7-Amino-4-methylcoumarin - CAS 26093-31-2 - Calbiochem

Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity.

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Synonym(s):

AMC, Coumarin 120, 7-Amino-4-methylcoumarin - CAS 26093-31-2 - Calbiochem

Empirical Formula (Hill Notation):

C₁₀H₉NO₂

CAS Number:

26093-31-2

Molecular Weight:

175.18

MDL number:

MFCD00006868

Quality Level

100

assay

≥98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
protect from light

color

yellow

solubility

DMSO: 10 mg/mL
acetone: 10 mg/mL
DMF: soluble

fluorescence

λ_ex 365-380 nm
λ_em 430-460 nm

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3

InChI key

GLNDAGDHSLMOKX-UHFFFAOYSA-N

General description

Reagent used to prepare fluorogenic 7-amino-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzymes (excitation maximum: ~365-380 nm; emission maximum: ~430-460 nm). Also useful as a reference standard in enzyme assays.

Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity. Useful as a reference standard in enzyme assays.

Packaging

10 mg in Plastic ampoule

Packaged under inert gas

Biochem/physiol Actions

Primary Target
Detection of proteolytic enzyme activity

Product does not compete with ATP.

Reversible: no

Cell permeable: no

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). DMF and DMSO stock solutions are stable for up to 6 months at -20°C. Acetone stock solutions are stable for up to 1 week at 4°C or -20°C.

Other Notes

Kanaoka, Y., et al. 1982. Chem. Pharm. Bull. 30, 1485.
Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)