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Sigma-Aldrich

5,5ʹ-Dithio-bis-(2-nitrobenzoic Acid) - CAS 69-78-3 - Calbiochem

Sulfhydryl reagent used to characterize reactive SH groups.

Synonym(s):
5,5ʹ-Dithio-bis-(2-nitrobenzoic Acid) - CAS 69-78-3 - Calbiochem, DTNB, Ellman’s Reagent
Empirical Formula (Hill Notation):
C14H8N2O8S2
CAS Number:
Molecular Weight:
396.35
MDL number:

Quality Level

assay

≥98% (titration)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

yellow

solubility

ethanol: 8 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

General description

Sulfhydryl reagent used to characterize reacting SH groups. Useful in photometric determination of thiols.
Sulfhydryl reagent used to characterize reactive SH groups. Useful in photometric determination of thiols.

Packaging

5 g in Glass bottle
Packaged under inert gas

Biochem/physiol Actions

Cell permeable: no
Primary Target
Useful in photometric determination of thiols
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Preparation Note

May require gentle heating for complete solubilization.

Other Notes

Ellman, G.L., et al. 1961. Biochem. Pharmacol.7, 88.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

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Certificate of Quality

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