474791

InSolution MG-132 - CAS 133407-82-6 - Calbiochem

InSolution MG-132, CAS 133407-82-6, is provided as a 10 mM solution in DMSO. Acts as a potent, reversible proteasome inhibitor (K_i = 4 nM).

• Synonym(s): Z-Leu-Leu-Leu-al, Carbobenzoxy-L-leucyl-L-leucyl-L-leucinal, Z-LLL-CHO, Proteasome Inhibitor XI, MG-132
• Empirical Formula (Hill Notation): C₂₆H₄₁N₃O₅
• CAS Number: 133407-82-6
• Molecular Weight: 475.62
• MDL number: MFCD00674886

Properties

• Quality Level: 100
• assay: ≥98% (HPLC)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: [H]C(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)OCc1ccccc1
• InChI: 1S/C26H41N3O5/c1-17(2)12-21(15-30)27-24(31)22(13-18(3)4)28-25(32)23(14-19(5)6)29-26(33)34-16-20-10-8-7-9-11-20/h7-11,15,17-19,21-23H,12-14,16H2,1-6H3,(H,27,31)(H,28,32)(H,29,33)/t21-,22-,23-/m0/s1
• InChI key: TZYWCYJVHRLUCT-VABKMULXSA-N

Description

Amino Acid Sequence

Z-Leu-Leu-Leu-al

General description

Potent, reversible, and cell-permeable proteasome inhibitor (K_i = 4 nM). Reduces the degradation of ubiquitin-conjugated proteins in mammalian cells and permeable stains of yeast by the 26S complex without affecting its ATPase or isopeptidase activities. Activates c-Jun N-terminal kinase (JNK1) and inhibits NF-κB activation (IC₅₀ = 3 µM).

Packaging

1, 5 mg in Plastic ampoule

Packaged under inert gas

Biochem/physiol Actions

Cell permeable: yes

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: 3 µM in inhibiting NF-κB activation

Target K_i: 4 nM as proteasome inhibitor

Warning

Toxicity: Irritant (B)

Physical form

A 10 mM (1 mg/210 µl) or (5mg/1050 µl) solution of MG-132 (Cat. No. 474790) in DMSO.

Other Notes

Meriin, A.B., et al. 1998. J. Biol. Chem.273, 6373.
Adams, J., and Stein, R. 1996. Ann. Rep. Med. Chem.31, 279.
Lee, D.H., and Goldberg, A.L. 1996. J. Biol. Chem.271, 27280.
Wiertz, E.J.H.J., et al. 1996. Cell84, 769.
Read, M.A., et al. 1995. Immunity2, 493.
Rock, K.L., et al. 1994. Cell78, 761.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): 188.6 °F - (Dimethylsulfoxide)
• Flash Point(C): 87 °C - (Dimethylsulfoxide)