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5.09228

Sigma-Aldrich

InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested

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Synonym(s):
InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested
Empirical Formula (Hill Notation):
C14H21N3O · 2HCl
CAS Number:
146986-50-7
Molecular Weight:
320.26

Assay

≥95% (HPLC)

Quality Level

100

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

storage temp.

−20°C

InChI

1S/C14H21N3O/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13/h6-12H,2-5,15H2,1H3,(H,16,17,18)/t10-,11?,12?/m1/s1

InChI key

IYOZTVGMEWJPKR-VOMCLLRMSA-N

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This Item
688001SCM075688000
InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested

Sigma-Aldrich

5.09228

InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested

InSolution Y-27632 Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.

Sigma-Aldrich

688001

InSolution Y-27632

ROCK Inhibitor (Y-27632) The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

Sigma-Aldrich

SCM075

ROCK Inhibitor (Y-27632)

Y-27632-CAS 331752-47-7-Calbiochem Y-27632A, CAS 331752-47-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.

Sigma-Aldrich

688000

Y-27632-CAS 331752-47-7-Calbiochem

form

liquid

form

liquid

form

liquid

form

solid

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles, protect from light

storage condition

OK to freeze, protect from light

storage condition

-

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

A 10 mM (2 mg/591 µL) sterile-filtered solution of Y-27632 in H₂O.
Ready to use, cell culture-tested, suitable for stem cell applications. Acts as a highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (Ki = 140 nM for p160ROCK). Also shown to improve survival of human stem cells especially during cell culture passage and manipulation.

Application

InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested, CAS 146986-50-7, is a 10 mM sterile-filtered solution of Y-27632 in H₂O. Acts as an inhibitor of Rho kinases (Ki = 140 nM for p160ROCK).

Biochem/physiol Actions

Primary Target
ROCK

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Physical form

A 10 mM (2 mg/591 µL) sterile-filtered solution of Y-27632 (Cat. No. 688000) in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Other Notes

Li X. et al, et. Al., 2009. Human Reproduction, 24, 580.
Chitaley, K., et al. 2001. Nat. Med.7, 119.
Davies, S.P., et al. 2000. Biochem. J.351, 95.
Narumiya, S., et al. 2000. Methods Enzymol.325, 273.
Hirose, M., et al. 1999. J. Cell Biol.141, 1625.
Maekawa, M., et al. 1999. Science.285, 895.
Uehata, M., et al. 1997. Nature.389, 990.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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