Merck
All Photos(1)

70666

Millipore

Ni-NTA His•Bind® Resin

Synonym(s):
nickel charged agarose, nickel affinity gel

form

slurry

Quality Level

manufacturer/tradename

Novagen®

storage condition

do not freeze

shipped in

ambient

storage temp.

2-8°C

General description

Ni-NTA His•Bind Resin is a high-performance Ni2+-charged agarose used for rapid one-step purification of proteins containing a His•Tag sequence by metal chelation chromatography. NTA chemistry minimizes metal leaching during purification and is compatible with up to 10 mM β-mercaptoethanol or 1 mM Tris(hydroxypropyl)phosphine (THP) for reduction of disulfide bonds. Ni-NTA His•Bind Resin has a binding capacity of 5–10 mg His•Tag fusion protein per ml resin.

Supplied as a 50% slurry; the quantity/pack is based on the amount of the settled resin.

Packaging

10 ml in Plastic ampoule
100, 25, 500 ml in Glass bottle

Warning

Toxicity: Flammable (J)

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Flame

Signal Word

Danger

Hazard Statements

Hazard Classifications

Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Hadas Bar-Joseph et al.
Reproductive biomedicine online, 41(2), 335-342 (2020-06-14)
Does recombinant pigment epithelium derived factor (PEDF) have potential in treating uterine fibroids? In-vitro models that used human leiomyoma and Eker rat uterine leiomyoma (ELT-3) cell lines. The ELT-3 cell line was used to examine cellular targets after adding recombinant
Yu-Xi Luo et al.
The EMBO journal, 39(7), e102008-e102008 (2020-03-03)
Deposition of H2A.Z in chromatin is known to be mediated by a conserved SWR1 chromatin-remodeling complex in eukaryotes. However, little is known about whether and how the SWR1 complex cooperates with other chromatin regulators. Using immunoprecipitation followed by mass spectrometry
Bao-Rui Zhao et al.
Journal of immunology (Baltimore, Md. : 1950), 204(3), 487-497 (2019-12-20)
Rapid synthesis and release of active antimicrobial peptides (AMPs) is an important strategy in innate immune. Processing of the precursor into the active form is a common posttranslational modification of AMPs in mammals. However, in invertebrates, the mechanism of AMP

Articles

YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast

YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast

Related Content

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service