Merck
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AB3279

Sigma-Aldrich

Anti-Hexokinase Type II Antibody

Chemicon®, from rabbit

Synonym(s):
HK II
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... HK2(3099)

Specificity

Reacts with the Type II isozyme of rat hexokinase (102 kDa). No reactivity with the Type I or Type III isozymes of rat hexokinase by immunoblot or ELISA. Reactivity with human and hamster Type II isozyme has been confirmed by immunoblotting, and reactivity with Type II isozyme from other mammalian species is expected.

Immunogen

Recombinant rat Type II hexokinase.

Application

Western Blot: 1:5,000-1:10,000.

Immunocytochemistry using indirect immunofluorescence.

ELISA

Optimal working dilutions must be determined by the end user.
Research Category
Metabolism
Research Sub Category
Enzymes & Biochemistry
Anti-Hexokinase Type II Antibody is an antibody against Hexokinase Type II for use in ELISA, IC & WB.

Target description

102 kDa

Physical form

Immunodepleted Serum
Format: Purified
Liquid. Partially Purified rabbit Serum. Antibodies reactive with the Type I isozyme were present in the original antiSerum; these were removed by affinity chromatography on a column of immobilized Type I isozyme, and the affinity-depleted Serum restored to original volume.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
insulin-responsive tissues such as skeletal muscle

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Enzymatic properties of the N- and C-terminal halves of human hexokinase II.
Keun Jae Ahn,Jongsun Kim,Mijin Yun,Jeon Han Park,Jong Doo Lee
Bmb Reports null
Yuya Nogami et al.
BMC medical imaging, 16, 31-31 (2016-04-27)
We report two cases of anisakiasis lesions that were initially suspected to be recurrence of gynecological cancer by positron emission tomography-computed tomography (PET-CT). Both cases were extragastrointestinal anisakiasis that is very rare. The first case was a patient with endometrial
Heidi Lyng et al.
BMC genomics, 7, 268-268 (2006-10-24)
A better understanding of the development of metastatic disease and the identification of molecular markers for cancer spread would be useful for the design of improved treatment strategies. This study was conducted to identify gene expressions associated with metastatic phenotypes
Wenshan Hao et al.
The Journal of biological chemistry, 285(17), 12647-12654 (2010-01-30)
Cancer cells constantly adapt to oxidative phosphorylation (OXPHOS) suppression resulting from hypoxia or mitochondria defects. Under the OXPHOS suppression, AMP-activated protein kinase (AMPK) regulates global metabolism adjustments, but its activation has been found to be transient. Whether cells can maintain
Chung-Ling Lu et al.
PloS one, 10(3), e0121046-e0121046 (2015-03-26)
A unique feature of cancer cells is to convert glucose into lactate to produce cellular energy, even under the presence of oxygen. Called aerobic glycolysis [The Warburg Effect] it has been extensively studied and the concept of aerobic glycolysis in

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