Merck
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AB3420-I

Sigma-Aldrich

Anti-Surfactant Protein A Antibody

from rabbit

Synonym(s):
Pulmonary surfactant-associated protein A1, PSP-A, PSPA, SP-A, SP-A1, 35 kDa pulmonary surfactant-associated protein, Alveolar proteinosis protein, Collectin-4
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

species reactivity (predicted by homology)

primate (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... SFTPA1(653509)
mouse ... Sftpd(20390)

General description

Pulmonary surfactant-associated protein A1 (SP-A1, SFTPA1, PSP-A, SP-A) binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. There are four main surfactant proteins, known as SP-A, B, C, and D. SP-A was the first surfactant protein to be identified, and is also the most abundant. Its molecular mass varies from 26-38 kDa. It can be found in an open or closed form depending on the other substances present in the system. Calcium ions produce the closed-bouquet form. SP-A is necessary for the production of tubular myelin, a lipid transport structure unique to the lungs. Genetic variations in SP-A are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF) and respiratory distress syndrome in premature infants (RDS).

Specificity

Other homologies: Uniprot shows no homology to Rat.
This antibody recognizes the C-type lectin domain of Surfactant Protein A.

Immunogen

Epitope: C-type lectin domain
KLH-conjugated linear peptide corresponding to the C-type lectin domain of human Surfactant Protein A.

Application

Detect SP-A using this rabbit polyclonal antibody, Anti-Surfactant Protein A Antibody validated for use in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Surfactant Protein A in human lung tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in human lung tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Surfactant Protein A in 10 µg of human lung tissue lysate.

Target description

~35 kDa observed

Linkage

Replaces: AB3420

Physical form

Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human lung tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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