Merck
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AB5535

Sigma-Aldrich

Anti-Sox9 Antibody

Chemicon®, from rabbit

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Synonym(s):
Anti-CMD1, Anti-CMPD1, Anti-SRA1, Anti-SRXX2, Anti-SRXY10
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

chicken, human, rat, mouse

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), sheep (based on 100% sequence homology), feline (based on 100% sequence homology), equine (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

ChIP: suitable (ChIP-seq)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

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This Item
AB5603MABC785ABE571
Anti-Sox9 Antibody Chemicon®, from rabbit

Sigma-Aldrich

AB5535

Anti-Sox9 Antibody

Anti-Sox2 Antibody Chemicon®, from rabbit

Sigma-Aldrich

AB5603

Anti-Sox2 Antibody

Anti-SOX9 Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABE571

Anti-SOX9 Antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

4B7.1, monoclonal

clone

polyclonal

species reactivity

chicken, human, rat, mouse

species reactivity

human, mouse

species reactivity

human

species reactivity

mouse

packaging

antibody small pack of 25 μg

packaging

antibody small pack of 25 μg

packaging

-

packaging

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

General description

Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

Specificity

Recognizes Sox9.

Immunogen

Epitope: C-terminal
KLH-conjugated linear peptide corresponding to the C-terminal sequence of human Sox9.

Application

Anti-Sox9 Antibody is a well characterized affinity purified Rabbit Polyclonal Antibody that reliably detects Transcription Factor Sox-9. This highly published antibody has been validated in IHC & WB.
Immunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected Sox9 in murine embryonic bone and adult cartilage tissue sections.
Western Blotting Analysis: An 1:500 dilution from a representative lot detected Sox9 in human PC3 prostate cancer cells and HepG2 hepatocytes.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Sox9 occupancy at target chromatin sites by ChIP using chromatin preparations from P1 post-natal mouse rib chondrocytes (Ohba, S., et al. (2015). Cell Rep. 12(2):229-243).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Sox9 occupancy at the Bmi promoter in Z/sox9tg but not in wild-type control mouse embryonic fibroblasts/MEFs (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315).
ChIP-sequencing (ChIP-seq) Analysis: A representative lot detected Sox9-targeted chromatin sites by a genome-wide ChIP-seq analysis using chromatin preparations from P1 post-natal mouse rib chondrocytes (Ohba, S., et al. (2015). Cell Rep. 12(2):229-243).
Immunofluorescence Analysis: A representative lot detected the accumulation of Sox9-positive oval cells by fluorescent immunohistochemistry staining of paraffin-embedded liver sections from transgenic mice treated with diethylnitrosamine to induce conditional liver HNF4a knockout (Saha, S.K., et al. (2014). Nature. 513(7516):110-114).
Immunofluorescence Analysis: Representative lots detected Sox9 immunoreactivity in paraffin-embedded mouse embryo sections by fluorescent immunohistochemistry (Carrasco, M., et al. (2012). J. Clin. Invest. 122(10):3504-3515; Sylva, M., et al. (2011). PLoS One. 6(8):e22616).
Immunofluorescence Analysis: Representative lots immunostained Müller glial cells in frozen mouse and chicken retina sections by fluorescent immunohistochemistry staining of (Muranishi, Y., and Furukawa, T. (2012). J. Biomed. Biotechnol. 2012:973140; Fischer, A.J., et al. (2011). Neuroscience. 178:250-260).
Immunocytochemistry Analysis: A representative lot detected the stem cell marker Sox9 by fluorescent immunocytochemistry staining of paraformaldehyde-fixed E-Cad/Lgr6+ human lung stem cells (HLSCs) clonally derived and passaged in culture (Oeztuerk-Winder, F., et al. (2012). EMBO J. 31(16):3431-3441).
Immunohistochemistry Analysis: A representative lot immunostained the supporting cells (Sertoli) of the seminiferous tubules by immunohistochemistry staining of paraffin-embedded mouse testis sections (O′Shaughnessy, P.J., et al. (2012). PLoS One. 7(4):e35136).
Immunohistochemistry Analysis: A representative lot detected Sox9 immunoreactivity in various formalin-fixed, paraffin-embedded human tumor tissue sections (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315).
Western Blotting Analysis: A representative lot detected the stem cell marker Sox9 in E-Cad/Lgr6+ human lung stem cells (HLSCs) clonally derived and passaged in culture (Oeztuerk-Winder, F., et al. (2012). EMBO J. 31(16):3431-3441).
Western Blotting Analysis: A representative lot detected upregulated Sox9 expression level in human colorectal cancer cell lines, HCT116, DLD1, and SW620 (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: An 1:2000 dilution of this antibody detected Sox9 in HepG2 cell lysate.

Target description

56-65 kDa

Linkage

Replaces: AB5809

Physical form

ImmunoAffinity Purified
Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide

Storage and Stability

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

Analysis Note

Control
Embryonic tissue, Adult chondrocytes.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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A novel type of glial cell in the retina is stimulated by insulin-like growth factor 1 and may exacerbate damage to neurons and Muller glia.
Fischer, AJ; Scott, MA; Zelinka, C; Sherwood, P
Glia null
P Bernstein et al.
Osteoarthritis and cartilage, 18(12), 1596-1607 (2010-10-05)
The use of mesenchymal stem cells (MSCs) for cartilage regeneration is hampered by lack of knowledge about the underlying molecular differences between chondrogenically stimulated chondrocytes and MSCs. The aim of this study was to evaluate differences in phenotype and gene
Sarah Roxana Herlofsen et al.
Tissue engineering. Part A, 17(7-8), 1003-1013 (2010-11-23)
We have used a disc-shaped self-gelling alginate hydrogel as a scaffold for in vitro chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells. The comparison of monolayer cells and alginate embedded cells with or without differentiation medium allowed us to
Notch signaling influences neuroprotective and proliferative properties of mature Muller glia.
Ghai, K; Zelinka, C; Fischer, AJ
The Journal of Neuroscience null
Heterogeneity of glia in the retina and optic nerve of birds and mammals.
Fischer, AJ; Zelinka, C; Scott, MA
Testing null

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