Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Recognizes an epitope on the outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT).
Tyrosine Hydroxylase purified from PC12 cells
Detect Tyrosine Hydroxylase using this Anti-Tyrosine Hydroxylase Antibody, clone LNC1 validated for use in IH, IH(P), IP & WB with more than 85 product citations.
A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.
A previous lot of this antibody was used in IP (Perez, 2002).
Optimal working dilutions and protocols must be determined by end user.
Research Sub Category
Neurotransmitters & Receptors
Neuronal & Glial Markers
Routinely evaluated by Western Blot on Mouse Brain lysates.
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.
Ascites mouse monoclonal IgG1κ fluid containing 3% BSA. Contains no preservative.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Human brain tissue, extracts from 3T3 cells treated with Forskolin (40 nM, 30 min).
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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