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MAB5268

Sigma-Aldrich

Anti-Chromogranin A Antibody, clone LK2H10

clone LK2H10, Chemicon®, from mouse

Synonym(s):
CgA, Pituitary Secretory Protein I
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

clone

LK2H10, monoclonal

species reactivity

human, porcine, pig, rat, monkey, mouse

should not react with

guinea pig, sheep, rabbit

manufacturer/tradename

Chemicon®

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... CHGA(1113)

General description

Chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Specificity

Chromogranin, an acidic protein having a molecular weight of about 68 kDa abides in the polypeptide-hormone-containing secretory granules of human endocrine tissues. Anti-chromogranin A can be used to stain most normal and neoplastic endocrine tissues containing secretory granules (exceptions include cells from the hypothalamus and posterior pituitary, which are known to contain secretory granules). The amount of staining is usually proportional to the number of secretory granules present (1).

Packaging

500 μg in 500 μL
500 μL

Application

Detect Chromogranin A using this Anti-Chromogranin A Antibody, clone LK2H10 validated for use in IH, IH(P) & WB.
Immunohistochemistry:
A 1-10 μg/mL concentration of a previous lot was used in IH.

Optimal working dilutions must be determined by end user.

Quality

Routinely evaluated by Western Blot on Mouse Pancreas lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Chromogranin A on 10 μg of Mouse Pancreas lysate.

Target description

50 kDa

Linkage

Replaces: CBL219

Physical form

Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.05M Borate buffered saline, 0.15M NaCl, pH 8.2 with 0.1% sodium azide.

Analysis Note

Control
Pancreas, adrenal gland, bowel and thyroid tissues Immunoblot: PC12 cell lysate, human pancreatic tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

nwg

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

Fausto J Rodriguez et al.
Brain pathology (Zurich, Switzerland), 19(4), 623-629 (2008-08-20)
Co-deletion of chromosome arms 1p and 19q, characteristic of oligodendroglial tumors, was recently found to be mediated by t(1;19)(q10;p10). To evaluate the prevalence of 1p19q co-deletion and t(1;19) in extraventricular neurocytomas (EVN), we studied tumors from 23 patients, including 13...
Specific endocrine tissue marker defined by a monoclonal antibody.
Lloyd, R V and Wilson, B S
Science (New York, N.Y.), 222, 628-630 (1983)
Malin Grönberg et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 56(9), 793-801 (2008-05-14)
Obestatin and ghrelin are two peptides derived from the same prohormone. It is well established that ghrelin is produced by endocrine cells in the gastric mucosa. However, the distribution of human obestatin immunoreactive cells is not thoroughly characterized. A polyclonal...
Mark C Markowski et al.
The Prostate, 78(13), 992-1000 (2018-06-01)
Loss or mutation of PTEN alleles at 10q23 in combination with 8q24 amplification (encompassing MYC) are common findings in aggressive, human prostate cancer. Our group recently developed a transgenic murine model of prostate cancer involving prostate-specific Pten deletion and forced...
Noèlia Téllez et al.
American journal of physiology. Endocrinology and metabolism, 311(3), E554-E563 (2016-07-14)
Limitations in β-cell regeneration potential in middle-aged animals could contribute to the increased risk to develop diabetes associated with aging. We investigated β-cell regeneration of middle-aged Wistar rats in response to two different regenerative stimuli: partial pancreatectomy (Px + V)...

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