Merck
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PS1023

Sigma-Aldrich

PhosphoDetect Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9)

liquid, clone C25A9, Calbiochem®

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

C25A9, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SMAD3(4088)

General description

Purified rabbit monoclonal antibody generated by immunizing rabbits with the specified immunogen and fusing splenocytes with an immortal cell line. Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425.
Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425 in HT-1080 cells treated with TGF-β1.
This PhosphoDetect Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) is validated for use in Immunoblotting, Immunoprecipitation for the detection of Smad3 (pSer423/425).

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 423/425 phosphorylation sites of Smad3, coupled to KLH.

Packaging

50 μL in Plastic ampoule

Application


Immunoblotting (1:1000)
Immunoprecipitation (1:50)

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5.

Other Notes

Attisano, L., et al. 2002. Science 296, 1646.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.
Does not cross-react with other Smad proteins. For immunoblotting incubate the membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of an anti-rabbit secondary antibody. The immunogen sequence is 100% conserved in mouse, rat, monkey, bovine, xenopus, and zebrafish Smad3, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificate of Analysis

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Certificate of Quality

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