SCM102

Cardiomyocyte Differentiation Medium

Name: Cardiomyocyte Differentiation Medium
Brand: MM

The Cardiomyocyte Differentiation Medium is available in a 500 mL format & has been optimized & validated for Cell Differentiation.

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About This Item

UNSPSC Code:

12352207

eCl@ss:

32160801

NACRES:

NA.75

Key Documents

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Quality Level

100

form

liquid

technique(s)

cell differentiation: suitable

input

sample type cardiac stem cell(s)

shipped in

dry ice

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This Item	SCM101	SCM017	SCM121
Sigma-Aldrich SCM102 Cardiomyocyte Differentiation Medium	Sigma-Aldrich SCM101 Cardiac Stem Cell Maintenance Medium	Sigma-Aldrich SCM017 ENStem-A Neuronal Differentiation Medium	Sigma-Aldrich SCM121 OsteoMAX-XF Differentiation Medium
form liquid	form liquid	form liquid	form liquid
technique(s) cell differentiation: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| stem cell: suitable	technique(s) cell culture \| stem cell: suitable
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
input sample type cardiac stem cell(s)	input sample type cardiac stem cell(s)	input sample type neural stem cell(s)	input -

General description

The Cardiomyocyte Differentiation Medium has been optimized and qualified for the preferential differentiation of freshly isolated cardiac stem cells derived from rodent origins to cardiomyocytes. Cardiac stem cells maintained in Cardiomyocyte Differentiation Medium for 12-15 days should differentiate into cardiomyocyte and express the appropriate markers, troponin I, desmin, and actinin.

Physical form

Cardiomyocyte Differentiation Medium is a proprietary formulation that contains fetal bovine serum.

Preparation Note

For long term storage, maintain the media at -20°C. Prior to initial use, thaw frozen media at 4°C overnight or until it has become completely equilibrated. Maintain thawed media at 2-8°C in the dark for up to one month.

Analysis Note

Each lot of medium is tested for the optimal differentiation of cardiac stem cells to cardiomyocytes. Sterility Testing: Negative

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The isolation and expansion of cardiac stem cells opens new opportunities to the feld of cardiac regenerative medicine. CSCs have recently been isolated from human and murine tissues based upon cell biomarkers (Sca-1, TERT, c-Kit, side population), demonstrating the presence of a non-circulating stem cell niche within the myocardium that is estimated to account for 11-14% of the total cell population1. These cells appear to be bi-potent in their capacity to form cardiomyocytes and vascular endothelial cells. Moreover, preliminary engraftment studies suggest that these cells are ideal candidates for future research on cardiac regeneration. However, the rarity of CSCs coupled with the complex isolation procedure is a signifcant challenge for the advancement of the field. o overcome this obstacle, we have developed an easy-to-use cell isolation kit capable of obtaining a high yield, pure population of CSCs. This advancement allows investigators to obtain a signifcantly greater number of CSCs for their studies without the need for time consuming, complex protocols and expensive cell sorting equipment.

CARDIOMYOCYTE DIFFERENTIATION MEDIUM

Poster: Cardiac Stem Cell Isolation

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