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03734927001

Roche

Taq DNA Polymerase, GMP Grade

≥13000 units/mg protein, >98% (SDS-PAGE), sufficient for ≤2,000 reactions, Difficult Templates/Specialty Enzymes PCR

Synonym(s):
polymerase, primer extension, dna amplification, pcr
Enzyme Commission number:

Quality Level

description

5 U/βl

assay

>98% (SDS-PAGE)

usage

sufficient for ≤2,000 reactions

specific activity

≥13000 units/mg protein

feature

Difficult Templates/Specialty Enzymes PCR
dNTPs included: no
hotstart: no

packaging

pkg of 1,000 U

manufacturer/tradename

Roche

parameter

72 °C optimum reaction temp.

application(s)

PCR: suitable

input

purified DNA

optimum pH

~9 (20 °C)

shipped in

dry ice

storage temp.

−20°C (−15°C to −25°C)

General description

Taq DNA Polymerase, GMP Grade, is manufactured under GMP conditions. It also fulfills the high quality and documentation requirements of research and development laboratories in the pharmaceutical and biotechnology industries. Taq DNA Polymerase, GMP Grade, gives high lot-to-lot consistency. Recombinant Taq DNA Polymerase (from Thermus aquaticus, recombinant E. coli), GMP Grade, is a highly processive 5′–3′ DNA polymerase that lacks 3′–5′ exonuclease activity. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 95 kD. Taq DNA Polymerase also accepts modified deoxyribonucleoside triphosphates as substrates for highly efficient DNA labeling with radionucleotides, digoxigenin, fluorescein, or biotin.

Application

  • Taq DNA Polymerase, GMP Grade, can be applied for:
  • PCR
  • RT- PCR
  • Other primer-extension reactions, such as sequencing and labeling

Packaging

1 kit containing 2 components

Unit Definition

One unit Taq DNA Polymerase, GMP Grade is defined as the amount of enzyme that incorporates 20 nmol of total deoxyribonucleoside-triphosphates into acid precipitable DNA within 60 minutes at +65 °C under the assay conditions stated in the package insert.

Unit Assay: Incubation buffer for assay on activated DNA:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM mercaptoethanol, 0.2% polydocanol, 0.2 mg/ml gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP, pH 8.3 (25 °C).

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi [α-32P]-dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at 65 °C for 60 min. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 5 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kit Components Only

Product No.
Description

  • Taq DNA Polymerase, GMP Grade 5 U/μl

  • PCR Buffer with MgCl<sub>2</sub> 10x concentrated

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

M W Pfaffl
Nucleic acids research, 29(9), e45-e45 (2001-05-09)
Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform
Yun Huang et al.
PloS one, 5(1), e8888-e8888 (2010-02-04)
We recently showed that enzymes of the TET family convert 5-mC to 5-hydroxymethylcytosine (5-hmC) in DNA. 5-hmC is present at high levels in embryonic stem cells and Purkinje neurons. The methylation status of cytosines is typically assessed by reaction with

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