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04743814001

Roche

Expand 20 kbPLUS PCR System, dNTPack

sufficient for ≤40 reactions, pkg of 200 U, suitable for PCR, dNTPs included

Enzyme Commission number:

usage

sufficient for ≤40 reactions

feature

Long Range PCR
dNTPs included
hotstart: no

packaging

pkg of 200 U

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

parameter

68 °C optimum reaction temp.

technique(s)

PCR: suitable

input

purified DNA

shipped in

dry ice

storage temp.

−20°C (−15°C to −25°C)

General description

The Expand 20 kbPLUS PCR System is composed of an enzyme mix containing thermostable Taq DNA polymerase and Tgo DNA polymerase, a thermostable DNA polymerase with proofreading activity. This powerful polymerase mixture and associated buffer system is designed to give a high yield of PCR product when fragments longer than 20 kb need to be amplified.
T/A cloning can also be performed.
The kit has control human genomic DNA and a set of control primers for amplifying a 23kb fragment. This test can be used to test template DNA and primer pair performance.
Use 3.75 U for a standard 50μL PCR.
Each dNTPack contains 10mM additive-free sodium salt nucleotides as a ready-to-use mix.
To obtain long PCR products, first evaluate length and purity of the DNA template. Handling all genomic DNA templates with care. Genomic DNA template should be larger than 50kb, as determined with agarose gel electrophoresis.

Application

Choose Expand 20 kbPLUS PCR System when amplifying genomic DNA fragments up to 35 kb. Expand 20 kbPLUS PCR System features a specifically optimized buffer and enzyme-blend mixture to amplify extra-long pieces of DNA (over 20 kb). Control primers and DNA produce fragments of 23 kb in length.
  • PCR
  • RT-PCR
  • Large-fragment amplification

Features and Benefits

  • Go the limit: The buffer and enzyme-blend amplify products over 20kb DNA.
  • Easy PCR monitoring: Human genomic DNA and human control β-globin primers amplify a 23kb fragment.
  • Test template quality: Control reagents also test template quality and primer pair performance.
  • Cost-effective: Use the convenient premixed solution of PCR grade dNTPs.

Packaging

1 kit containing 7 components

Quality

Each lot of Expand 20kbPLUS PCR System is function-tested in PCR using human genomic DNA and specific primers for β-globin to amplify a 23kb fragment.

Unit Definition

Volume Activity: 5 U/μl

Storage and Stability

Store supplied human control DNA at 2–8 °C, all other components at -15 to -25°C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Expand is a trademark of Roche

Kit Components Only

Product No.
Description

  • Expand 20 kbPLUS Enzyme Mix

  • Expand 20 kbPLUS Reaction Buffer, with 27.5 mM MgCl2 10x concentrated

  • MgCl2 Stock Solution 25 mM

  • Human Genomic DNA

  • Human β-globin Control Primer forward, (HβG forward)

  • Human β-globin Control Primer reverse, (HβG reverse)

  • PCR Nucleotide Mix

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Articles

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service