Merck
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11207733910

Roche

Anti-Digoxigenin-POD, Fab fragments

from sheep

Synonym(s):
digoxigenin, anti-digoxigenin

Quality Level

biological source

sheep

conjugate

peroxidase conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized (clear, brown solution after reconstitution)

packaging

pkg of 150 U

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

General description

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from an anti-digoxigenin antibody, conjugated with horse-radish peroxidase (POD). Anti-Digoxigenin-POD, Fab fragments is useful for the detection of digoxigenin-labeled compounds.

Specificity

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Application

Use Anti-Digoxigenin-POD, Fab fragments for the detection of digoxigenin-labeled compounds using:
  • Dot blot
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot

Preparation Note

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.

Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline: Dot blot: 150 mU/ml
  • ELISA: 50 to 150 mU/ml
  • Immunohistocytochemistry: 250 to 500 mU/ml
  • In situ hybridization: 1.5 to 7.5 U/ml
  • Southern blot: 150 mU/ml
  • Western blot: 250 to 500 mU/ml

Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Reconstitution

Add 1 ml double-distilled water to a final concentration of 150 U/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Hande Tunbak et al.
eLife, 9 (2020-05-06)
The zebrafish was used to assess the impact of social isolation on behaviour and brain function. As in humans and other social species, early social deprivation reduced social preference in juvenile zebrafish. Whole-brain functional maps of anti-social isolated (lonely) fish
X Peng et al.
Leukemia, 29(12), 2355-2365 (2015-06-25)
Controlled self-renewal and differentiation of hematopoietic stem/progenitor cells (HSPCs) are critical for vertebrate development and survival. These processes are tightly regulated by the transcription factors, signaling molecules and epigenetic factors. Impaired regulations of their function could result in hematological malignancies.
Sarah E Mercer et al.
PloS one, 7(12), e52375-e52375 (2013-01-10)
The inability to functionally repair tissues that are lost as a consequence of disease or injury remains a significant challenge for regenerative medicine. The molecular and cellular processes involved in complete restoration of tissue architecture and function are expected to
Yutaka Daido et al.
Developmental biology, 392(1), 117-129 (2014-05-07)
The vertebrate retina contains two types of photoreceptor cells, rods and cones, which use distinct types of opsins and phototransduction proteins. Cones can be further divided into several subtypes with differing wavelength sensitivity and morphology. Although photoreceptor development has been
Charless C Fowlkes et al.
PLoS genetics, 7(10), e1002346-e1002346 (2011-11-03)
Differences in the level, timing, or location of gene expression can contribute to alternative phenotypes at the molecular and organismal level. Understanding the origins of expression differences is complicated by the fact that organismal morphology and gene regulatory networks could

Articles

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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