Merck
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11699695001

Roche

ATP Bioluminescence Assay Kit CLS II

sufficient for 800 assays (tubes), kit of 1 (2 components), suitable for detection

Synonym(s):
bioluminescence assay

assay

>98%

Quality Level

usage

sufficient for 800 assays (tubes)

packaging

kit of 1 (2 components)

manufacturer/tradename

Roche

parameter

15-25 °C optimum reaction temp.

application(s)

detection

shipped in

dry ice

storage temp.

−20°C

General description

ATP bioluminescence assay is a sophisticated micro count method. It works based on the capability of fireflies to generate its glowing cold light. Rise in proliferation for all cytohexdrin concentrations can be determined by ATP bioluminescence assay.

Specificity

The ATP Bioluminescence Assay Kit CLS II is specially developed for applications in which constant light signals are required for kinetic studies of enzymes and metabolic studies, or if coupled enzymatic assays are applied. If ATP determinations are performed manually, the CLS Kit provides high reproducibility due to the constant signal generation.
The sensitivity of the kit is reduced by a factor of 10, compared to the ATP Bioluminescence Assay Kit HS II, which is recommended for determinations that are in the high-sensitivity range. The ATP Bioluminescence Assay Kit HS II also contains an efficient cell lysis reagent, and can be used for the detection of ATP in microorganisms or animal cells.

Application

ATP Bioluminescence Assay Kit CLS II has been used in the determination of the ATP synthetic activity of the H+-ATP synthase, ATP bioluminescent assay and intracellular measurement of ATP levels.
The ATP Bioluminescence Assay Kit CLS II is optimized for easy use in tube luminometers and microplate-format luminometers. The kit exhibits a constant light signal that is sustained for several minutes. The kit is well suited for kinetic studies and ATP determinations in coupled enzymatic reactions.
For more highly sensitive ATP determinations, use the ATP Bioluminescence Assay Kit HS  II.

Packaging

1 kit containing 2 components.

Preparation Note

Working concentration: Working range: 10-6 to 10-11 M ATP
Working solution: Preparation of working solutions

Luciferase Reagent
Dissolve the whole content of one bottle by carefully adding 10 ml of double-dist. water. Incubate for five minutes at 0 to 4 °C without stirring or shaking. Mix for a homogeneous solution by carefully rotating the bottle. Do not shake. The reagent is stable for one day at 15 to 25 °C or for one week when stored at 0 to 4 °C. However, set up a standard curve each day, because a slightly loss of light activity occurs during this time (approx. 20% after 5 days).

ATP Standard
Each bottle contains approx. 10 mg ATP (> 98% purity; Mr 605.2). The exact amount of ATP is determined individually for each lot as indicated on the label. Dissolve the content of one bottle by addition of the appropriate volume of double-dist. water to get a final concentration of 10 mg/ml or 16.5 mM, respectively (e.g., 960 μl to 9.60 mg ATP).

Reconstitution

Roche recommends reconstituting Luciferase Reagent in water and not in buffer.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Luciferase Reagent, lyophilized (8)

  • ATP Standard, lyophilized (for calibration) (4)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Role of glucose metabolism and ATP in maintaining PINK1 levels during Parkin-mediated mitochondrial damage responses.
Lee S, et al.
The Journal of Biological Chemistry, 290(2), 904-917 (2014)
Intracellular assessment of ATP levels in Caenorhabditis elegans.
Palikaras K and Tavernarakis N
Bio-protocol, 6(23), e22048-e22048 (2016)
Bioluminescence & Chemiluminescence: Progress & Current Applications (2002)
Schuyler Lee et al.
The Journal of biological chemistry, 290(2), 904-917 (2014-11-19)
Mutations in several genes, including PINK1 and Parkin, are known to cause autosomal recessive cases of Parkinson disease in humans. These genes operate in the same pathway and play a crucial role in mitochondrial dynamics and maintenance. PINK1 is required
Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems: Biosensors, Recognition Receptors, and Microsystem (2008)

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